Metabolomics insights and bioprospection of Polygonatum verticillatum: An important dietary medicinal herb of alpine Himalaya

Abstract

Polygonatum verticillatum is one of the least explored plant of genus Polygonatum, having immense utility in food and medicine. The current study was designed to investigate and compare the metabolite diversity, including the nutritional potential of rhizomatous and aerial parts (leaves and fruits) of Polygonatum verticillatum. Comprehensive metabolites through UPLC-PDA-ESI/MS were tentatively identified 38, 31, and 16 compounds while 342, 414, and 314 were identified through METLIN database in leaves, fruits, and rhizomes, respectively. Total phenolic, flavonoid, protein, carbohydrate, and fat content were compared and found in the range of 24.50-27.64 and 101.40-109.50, 99-100, 58-200, 5-56 µg/mg, respectively, while antioxidant activity was highest in fruits (IC_50DPPH 456.30 µg/ml). Polyphenols, essential amino acids, and macro & micronutrients were estimated in all the parts of P. verticillatum. Leaves contained the highest number of targeted polyphenols and amino acids, followed by fruits and rhizomes. Statistical analysis (Venn-diagram, Heatmap, stacked charts, PCA, PCoA) has visualized that all parts have similar nutritional potential and chemical diversity. The current finding unleashed the possibilities of utilizing aerial parts instead of rhizomes that would save this plant from overexploitation.

Keywords: Flavonoid; METLIN; Metabolomics; Polygonatum verticillatum; Saponin; UPLC-ESI-MS/MS.