Ribosomal proteins of yeast strains carrying mutations which affect the efficiency of nonsense suppression

Abstract

We have examined the ribosomal proteins of strains of Saccharomyces cerevisiae which differ in the efficiency with which ochre nonsense mutations are suppressed. The strains in which ochre suppression is poor were [psi]- or carried antisuppressor mutations; those in which suppression was highly efficient were [psi]+ or carried allosuppressor mutations. The ribosomal proteins of these strains, as judged by two-dimensional polyacrylamide gel electrophoresis, were indistinguishable from those of wild-type.