Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 May;11(3):458-468.
doi: 10.34172/apb.2021.053. Epub 2020 Aug 17.

Radachlorin-Containing Microparticles for Photodynamic Therapy

Sergey Petrovich Krechetov  1 , Anastasia Maksimovna Miroshkina  2 , Maria Nikolaevna Yakovtseva  1 , Elizaveta Nikitichna Mochalova  1 , Andrey Vadimovich Babenyshev  1 , Ivan Vladimirovich Maslov  3 , Alexander Alexandrovich Loshkarev  4 , Ivan Ivanovich Krasnyuk  2
Affiliations

Radachlorin-Containing Microparticles for Photodynamic Therapy

Sergey Petrovich Krechetov et al. Adv Pharm Bull. 2021 May.

Abstract

Purpose: Reducing the undesirable systemic effect of photodynamic therapy (PDT) can be achieved by incorporating a photosensitizer in microparticles (MPs). This study is devoted to the preparation of biocompatible biodegradable MPs with the inclusion of the natural photosensitizer Radachlorin (RС) and an assessment of the possibility of their use for PDT. Methods: RC-containing MPs (RС MPs) with poly(lactic-co-glycolic acid) copolymer (PLGA) matrix were prepared by a double emulsion solvent evaporation methods. The size and morphology of RC MPs were surveyed using scanning electron microscopy, confocal laser scanning microscopy, and dynamic light scattering. The content of RC, its release from RC MPs, and singlet oxygen generation were evaluated by the optical spectroscopy. Cellular uptake and cytotoxic photodynamic effect of RC MPs were investigated with in vitro assays. Results: The average diameter of the prepared RC MPs was about 2-3 μm. The RC MPs prepared by the water/oil/oil method had a significantly higher inclusion of RC (1.74 μg/mg) then RC MPs prepared by the water/oil/water method (0.089 μg/mg). Exposure of the prepared RC MPs to PDT light radiation was accompanied by the singlet oxygen generation and a cytotoxic effect for tumor cells. The release of the RC from the RC MPs was prolonged and lasted at least two weeks. Conclusion: PLGA RC MPs were found to cause a photoactivated cytotoxic effect for tumor cells and can be used for local application in PDT of tumors.

Keywords: Drug delivery systems; Microparticles; Photodynamic therapy; Photosensitizing agents; Radachlorin; Tumor cell line.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Figure 2
Figure 2
Figure 3
Figure 3
Figure 4
Figure 4
Figure 5
Figure 5
Figure 6
Figure 6
Figure 7
Figure 7
Figure 8
Figure 8
Figure 9
Figure 9
See this image and copyright information in PMC

References

    1. Falzone L, Salomone S, Libra M. Evolution of cancer pharmacological treatments at the turn of the third millennium. Front Pharmacol. 2018;9:1300. doi: 10.3389/fphar.2018.01300. - DOI - PMC - PubMed
    1. Gerhards NM, Rottenberg S. New tools for old drugs: functional genetic screens to optimize current chemotherapy. Drug Resist Updat. 2018;36:30–46. doi: 10.1016/j.drup.2018.01.001. - DOI - PMC - PubMed
    1. Nair PR. Delivering Combination chemotherapies and targeting oncogenic pathways via polymeric drug delivery systems. Polymers (Basel) 2019;11(4):630. doi: 10.3390/polym11040630. - DOI - PMC - PubMed
    1. Pan J, Rostamizadeh K, Filipczak N, Torchilin VP. Polymeric co-delivery systems in cancer treatment: an overview on component drugs’ dosage ratio effect. Molecules. 2019;24(6) doi: 10.3390/molecules24061035. - DOI - PMC - PubMed
    1. Aftab S, Shah A, Nadhman A, Kurbanoglu S, Aysıl Ozkan S, Dionysiou DD. et al. Nanomedicine: an effective tool in cancer therapy. Int J Pharm. 2018;540(1-2):132–49. doi: 10.1016/j.ijpharm.2018.02.007. - DOI - PubMed

LinkOut - more resources