Miniaturized Vascularized Bleeding Model of Hemostasis

Elaissa Trybus Hardy^{1

2

3}, Yumiko Sakurai^{1

2}, Wilbur A Lam^{4

5

6}

Affiliations

¹ Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA.
² Department of Pediatrics, Division of Pediatric Hematology/Oncology, Emory University School of Medicine, Atlanta, GA, USA.
³ Aflac Cancer and Blood Disorders Center of Children's Healthcare of Atlanta, Atlanta, GA, USA.
⁴ Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA. wilbur.lam@emory.edu.
⁵ Department of Pediatrics, Division of Pediatric Hematology/Oncology, Emory University School of Medicine, Atlanta, GA, USA. wilbur.lam@emory.edu.
⁶ Aflac Cancer and Blood Disorders Center of Children's Healthcare of Atlanta, Atlanta, GA, USA. wilbur.lam@emory.edu.

PMID: 34520012
DOI: 10.1007/978-1-0716-1693-2_10

Miniaturized Vascularized Bleeding Model of Hemostasis

Elaissa Trybus Hardy et al. Methods Mol Biol. 2022.

. 2022:2373:159-175.

doi: 10.1007/978-1-0716-1693-2_10.

Authors

Elaissa Trybus Hardy^{1

2

3}, Yumiko Sakurai^{1

2}, Wilbur A Lam^{4

5

6}

Affiliations

¹ Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA.
² Department of Pediatrics, Division of Pediatric Hematology/Oncology, Emory University School of Medicine, Atlanta, GA, USA.
³ Aflac Cancer and Blood Disorders Center of Children's Healthcare of Atlanta, Atlanta, GA, USA.
⁴ Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, USA. wilbur.lam@emory.edu.
⁵ Department of Pediatrics, Division of Pediatric Hematology/Oncology, Emory University School of Medicine, Atlanta, GA, USA. wilbur.lam@emory.edu.
⁶ Aflac Cancer and Blood Disorders Center of Children's Healthcare of Atlanta, Atlanta, GA, USA. wilbur.lam@emory.edu.

PMID: 34520012
DOI: 10.1007/978-1-0716-1693-2_10

Abstract

This chapter describes the development of a poly(dimethylsiloxane)-based microfluidic platform that is able to holistically assess and visualize the entire hemostatic process in vitro. The microfluidic platform includes (1) integration of intact endothelium, (2) physiological flow conditions, (3) controlled mechanical injury to study global hemostatic potential of a patient's whole blood samples in a microvascular model and for dissecting pathophysiologic mechanisms of diseases.

Keywords: Endothelium; Hemostasis; Injury model; Microfluidics; Microvasculature.

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References

1. Sakurai Y et al (2018) A microengineered vascular bleeding model that integrates the principal components of hemostasis. Nat Commun 9:509 - DOI
1. Myers DR et al (2012) Endothelialized microfluidics for studying microvascular interactions in hematologic diseases. J Vis Exp 22:3958
1. Occhetta P, Visone R, Rasponi M (2017) High-throughput microfluidic platform for 3D cultures of mesenchymal stem cells. Methods Mol Biol 1612:303–323 - DOI
1. Casquillas GV, Le Berre M, Terriac E, Bertholle F. https://www.elveflow.com/microfluidic/PDMS%20thickness%20VS%20spin%20spe... . Accessed 5 October 2020

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Miniaturized Vascularized Bleeding Model of Hemostasis

Affiliations

Miniaturized Vascularized Bleeding Model of Hemostasis

Authors

Affiliations

Abstract

References

Publication types

MeSH terms

Substances

Grants and funding

LinkOut - more resources

Full Text Sources