Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Dec;193(12):3949-3969.
doi: 10.1007/s12010-021-03660-3. Epub 2021 Sep 16.

Genome Mining of Three Plant Growth-Promoting Bacillus Species from Maize Rhizosphere

Oluwaseyi Samuel Olanrewaju  1 , Modupe Stella Ayilara  1 , Ayansina Segun Ayangbenro  1 , Olubukola Oluranti Babalola  2
Affiliations

Genome Mining of Three Plant Growth-Promoting Bacillus Species from Maize Rhizosphere

Oluwaseyi Samuel Olanrewaju et al. Appl Biochem Biotechnol. 2021 Dec.

Abstract

Bacillus species genomes are rich in plant growth-promoting genetic elements. Bacillus subtilis and Bacillus velezensis are important plant growth promoters; hence, to further improve their abilities, the genetic elements responsible for these traits were characterized and reported. Genetic elements reported include those of auxin, nitrogen fixation, siderophore production, iron acquisition, volatile organic compounds, and antibiotics. Furthermore, the presence of phages and antibiotic-resistant genes in the genomes are reported. Pan-genome analysis was conducted using ten Bacillus species. From the analysis, pan-genome of Bacillus subtilis and Bacillus velezensis are still open. Ultimately, this study brings an insight into the genetic components of the plant growth-promoting abilities of these strains and shows their potential biotechnological applications in agriculture and other relevant sectors.

Keywords: Bacillus; Biosynthetic gene clusters; Comparative genomics; Functional genomics; Pan-genome analysis; Plant growth-promoting bacteria.

PubMed Disclaimer

Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
Schematic representation and general characteristics of the three Bacillus spp. (a) BSA1. (b) BSA29. (c) BVA3
Fig. 2
Fig. 2
Frequency distribution of gene categories in each genome
Fig. 3
Fig. 3
Bacteriocins detection from BAGEL 4
Fig. 4
Fig. 4
Presence of antimicrobial resistance genes in the genomes of the three isolates
Fig. 5
Fig. 5
Remnants of bacteriophage regions. The boxes are color-coded with the legend pasted below the figure to show their potential functions
Fig. 6
Fig. 6
Pan- and core-genomes based on the number of sequenced genomes
Fig. 7
Fig. 7
Phylogenetics of the genomes based on the (a) core- and (b) pan-genomes
Fig. 8
Fig. 8
Average Nucleotide Identity (ANI) demonstrating nucleotide-level genomic similarity between the coding regions of indicated Bacillus subtilis and Bacillus velezensis genomes. Pairwise comparisons for all 10 genomes were computed using the OAT Program
See this image and copyright information in PMC

References

    1. Abd El-Daim IA, Bejai S, Meijer J. Bacillus velezensis 5113 induced metabolic and molecular reprogramming during abiotic stress tolerance in wheat. Scientific Reports. 2019;9:1–18. - PMC - PubMed
    1. Adeniji AA, Aremu OS, Babalola OO. Selecting lipopeptide-producing, Fusarium-suppressing Bacillus spp.: Metabolomic and genomic probing of Bacillus velezensis NWUMFkBS10.5. Microbiology Open. 2019;8:e00742. - PMC - PubMed
    1. Ajilogba CF, Babalola OO. GC–MS analysis of volatile organic compounds from Bambara groundnut rhizobacteria and their antibacterial properties. World Journal of Microbiology and Biotechnology. 2019;35:83. - PMC - PubMed
    1. Alcock BP, Raphenya AR, Lau TTY, Tsang KK, Bouchard M, Edalatmand A, Huynh W, Nguyen A-LV, Cheng AA, Liu S, Min SY, Miroshnichenko A, Tran H-K, Werfalli RE, Nasir JA, Oloni M, Speicher DJ, Florescu A, Singh B, Faltyn M, Hernandez-Koutoucheva A, Sharma AN, Bordeleau E, Pawlowski AC, Zubyk HL, Dooley D, Griffiths E, Maguire F, Winsor GL, Beiko RG, Brinkman FSL, Hsiao WWL, Domselaar GV, McArthur AG. CARD 2020: Antibiotic resistome surveillance with the comprehensive antibiotic resistance database. Nucleic Acids Research. 2019;48:D517–D525. - PMC - PubMed
    1. Alori, E. T., Dare, M. O., & Babalola, O. O. (2017). Microbial inoculants for soil quality and plant health. Sustainable Agriculture Reviews,281–30710.1007/978-3-319-48006-0_9

LinkOut - more resources