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. 2021 Dec:298:114281.
doi: 10.1016/j.jviromet.2021.114281. Epub 2021 Sep 13.

Comparative study of different SARS-CoV-2 diagnostic techniques

L Vallejo  1 , M Martínez-Rodríguez  1 , M J Nieto-Bazán  1 , A Delgado-Iribarren  2 , E Culebras  3
Affiliations

Comparative study of different SARS-CoV-2 diagnostic techniques

L Vallejo et al. J Virol Methods. 2021 Dec.

Abstract

The rapid spread of SARS-CoV-2 led to the necessity of developing diagnostic tests for rapid virus detection. Many commercial platforms have appeared and have been approved for this purpose. In this study, 95 positive and 5 negative retrospective samples were analyzed by 4 different commercial RT-qPCR kits (TaqMan 2019nCoV Assay, Allplex™SARS-COV-2 Assay, FTD SARS-COV-2 Assay and qCOVID-19). The Hologic Aptima SARS-COV-2 and the Clart-COVID-19 system were also tested. serial dilutions of SARS-COV-2 standard control were included for sensitivity analysis. Among the qPCR tested qCOVID19 and Allplex™SARS-COV-2 Assay were both able to detect all the clinical samples included in the study. All four qPCR evaluated showed high sensitivity for samples with Ct<33. Clart-COVID-19 microarrays detected all samples and controls used in this study whereas Hologic Aptima Panther failed with one of the clinical samples. However, the main problem with this system was the number of invalidated samples despite avoiding the use of medium with guanidine isothiocyanate as recommended by the manufacturer. All the techniques tested were of value for SARS-CoV-2 detection.

Keywords: Microarrays; RT-qPCR; SARS-CoV-2 detection; TMA.

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Conflict of interest statement

The authors report no declarations of interest.

Figures

Fig. 1
Fig. 1
Efficiency comparison of four commercial RT-qPCR according to N probe cycle threshold. Ninety-five positive clinical samples were used for the analysis. TaqMan 2019nCoV Assay (Thermofisher), qCOVID-19 (Genómica), FTD SARS-CoV-2 Assay (Siemens), Allplex™SARS-CoV-2 (Seegene).
Fig. 2
Fig. 2
Comparison of the different probes of TaqMan 2019nCoV Assay (Thermofisher) and Allplex™SARS-CoV-2 (Seegene). ORF, probe for the open reading frame 1a and b; S, probe for spike protein of SARS-CoV-2; N, nucleocapsid protein; E, probe for E-gene, gene encoding the envelope protein; RdRP probe for the RNA-dependent RNA polymerase of SARS-CoV-2.
See this image and copyright information in PMC

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