Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Sep 17;15(9):e0009739.
doi: 10.1371/journal.pntd.0009739. eCollection 2021 Sep.

Trypanosome SL-RNA detection in blood and cerebrospinal fluid to demonstrate active gambiense human African trypanosomiasis infection

Ipos Ngay Lukusa  1 , Nick Van Reet  2 , Dieudonné Mumba Ngoyi  1 , Erick Mwamba Miaka  3 , Justin Masumu  1 , Pati Patient Pyana  1 , Wilfried Mutombo  3 , Digas Ngolo  3 , Vincent Kobo  3 , Felix Akwaso  3 , Médard Ilunga  3 , Lewis Kaninda  3 , Sylvain Mutanda  3 , Dieudonné Mpoyi Muamba  3 , Olaf Valverde Mordt  4 , Antoine Tarral  4 , Sandra Rembry  4 , Philippe Büscher  2 , Veerle Lejon  5
Affiliations

Trypanosome SL-RNA detection in blood and cerebrospinal fluid to demonstrate active gambiense human African trypanosomiasis infection

Ipos Ngay Lukusa et al. PLoS Negl Trop Dis. .

Abstract

Background: Spliced Leader (SL) trypanosome RNA is detectable only in the presence of live trypanosomes, is abundant and the Trypanozoon subgenus has a unique sequence. As previously shown in blood from Guinean human African trypanosomiasis (HAT) patients, SL-RNA is an accurate target for diagnosis. Detection of SL-RNA in the cerebrospinal fluid (CSF) has never been attempted. In a large group of Congolese gambiense HAT patients, the present study aims i) to confirm the sensitivity of SL-RNA detection in the blood and; ii) to assess the diagnostic performance of SL-RNA compared to trypanosome detection in CSF.

Methodology/principal findings: Blood and CSF from 97 confirmed gambiense HAT patients from the Democratic Republic of Congo were collected using PAXgene blood RNA Tubes. Before RNA extraction, specimens were supplemented with internal extraction control RNA to monitor the extraction, which was performed with a PAXgene Blood RNA Kit. SL-RNA qPCR was carried out with and without reverse transcriptase to monitor DNA contamination. In blood, 92/97 (94.8%) HAT patients tested SL-RNA positive, which was significantly more than combined trypanosome detection in lymph and blood (78/97 positive, 80.4%, p = 0.001). Of 96 CSF RNA specimens, 65 (67.7%) were SL-RNA positive, but there was no significant difference between sensitivity of SL-RNA and trypanosome detection in CSF. The contribution of DNA to the Cq values was negligible. In CSF with normal cell counts, a fraction of SL-RNA might have been lost during extraction as indicated by higher internal extraction control Cq values.

Conclusions/significance: Detection of SL-RNA in blood and CSF allows sensitive demonstration of active gambiense HAT infection, even if trypanosomes remain undetectable in blood or lymph. As this condition often occurs in treatment failures, SL-RNA detection in blood and CSF for early detection of relapses after treatment deserves further investigation.

Trial registration: This study was an integral part of the diagnostic trial "New Diagnostic Tools for Elimination of Sleeping Sickness and Clinical Trials: Early tests of Cure" (DiTECT-HAT-WP4, ClinicalTrials.gov Identifier: NCT03112655).

PubMed Disclaimer

Conflict of interest statement

The authors have declared that no competing interests exist.

Figures

Fig 1
Fig 1. Cq values of RT-qPCR SL-RNA detection in blood and CSF of gambiense HAT patients.
The results are combined with RT-qPCR for internal extraction control (IEC), according the disease stage (E early, I Intermediate and L late stage), parasitological test result in blood and lymph (P), parasitological test result in CSF (C), and CSF WBC count/μl. + trypanosome presence;—absence of trypanosomes. ND not determined.
Fig 2
Fig 2. Cq values of the internal extraction control RT-qPCR in CSF in function of the CSF white blood cell count/μl.
Blue dots: trypanosomes in CSF. Red dots: no trypanosomes in CSF.
See this image and copyright information in PMC

References

    1. WHO, 2013. Control and surveillance of human African trypanosomiasis: report of a WHO Expert Committee. Geneva: World Health Organization; 2013. 237 p. (WHO technical report series). - PubMed
    1. Chappuis F, Loutan L, Simarro P, Lejon V, Buscher P. Options for Field Diagnosis of Human African Trypanosomiasis. Clin Microbiol Rev. 2005;18(1):133–46. doi: 10.1128/CMR.18.1.133-146.2005 - DOI - PMC - PubMed
    1. Büscher P, Deborggraeve S. How can molecular diagnostics contribute to the elimination of human African trypanosomiasis? Expert Rev Mol Diagn. 2015;15(5):607–15. doi: 10.1586/14737159.2015.1027195 - DOI - PubMed
    1. Deborggraeve S, Lejon V, Ekangu RA, Mumba Ngoyi D, Pati Pyana P, Ilunga M, et al.. Diagnostic Accuracy of PCR in gambiense Sleeping Sickness Diagnosis, Staging and Post-Treatment Follow-Up: A 2-year Longitudinal Study. PLoS Negl Trop Dis. 2011;5(2):e972. doi: 10.1371/journal.pntd.0000972 - DOI - PMC - PubMed
    1. Ilboudo H, Camara O, Ravel S, Bucheton B, Lejon V, Camara M, et al.. Trypanosoma brucei gambiense Spliced Leader RNA Is a More Specific Marker for Cure of Human African Trypanosomiasis Than T. b. gambiense DNA. J Infect Dis. 2015;212(12):1996–8. doi: 10.1093/infdis/jiv337 - DOI - PubMed

Publication types

MeSH terms

Associated data