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. 2021 Jun;13(3):319-324.
doi: 10.18502/ijm.v13i3.6394.

Serological and molecular investigation of human brucellosis in participants of Famenin brucellosis cohort study, Hamadan, Iran

Maryam Adabi  1 , Manoochehr Karami  2   3 , Fariba Keramat  1 , Mohammad Yousef Alikhani  1 , Somaye Bakhtiari  1
Affiliations

Serological and molecular investigation of human brucellosis in participants of Famenin brucellosis cohort study, Hamadan, Iran

Maryam Adabi et al. Iran J Microbiol. 2021 Jun.

Abstract

Background and objectives: Brucella is an intracellular pathogen that causes brucellosis in humans and animals. This study aimed to assess the results of brucellosis seroprevalence among participants of the Famenin brucellosis cohort with molecular investigation technique and determine Brucella-approved species.

Materials and methods: Following the first phase of the Famenin brucellosis cohort in 2016 which investigated the seroprevalence of brucellosis among 2367 participants in Famenin city, a total of 575 people including all seropositive and some seronegative people were examined again by wright serological tests in 2019. The PCR assay was accomplished on all cases that have wright titers ≥ 1/20 for tracing Brucella DNA using BCSP31 target gene and IS711 locus.

Results: Out of 575 studied cases, 145 people had wright titers ≥ 1/20. The PCR reactions of these 145 blood samples were positive in 63/145 (43.44%) tested samples using primers (B4/B5) for Brucella genus detection. In the second PCR assay using specific-primers for Brucella abortus and Brucella melitensis, 18/63 (28.57%) of the samples were diagnosed as B. abortus, and 18/63 (28.57%) were diagnosed as B. melitensis.

Conclusion: In this study, using the selected specific genes for the diagnosis of Brucella in the genus and species levels, the PCR technique was evaluated as a promising method for the rapid and safe detection of brucellosis besides the serological test for more accurate detection of brucellosis especially in cases that are not definitive.

Keywords: Brucella abortus; Brucella melitensis; Brucellosis; Polymerase chain reaction; Serological test.

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Figures

Fig. 1.
Fig. 1.
The PCR assay for detection of Brucella genus DNA. M: 1 kb Ladder, Lane 1: Negative control (physiological serum), Lane 2: Positive control (B. abortus ATTCC 23455), 3–5: Samples with Wright titer ≥1/20, 6 and 7: samples with no Wright titer
Fig. 2.
Fig. 2.
The PCR assay for the detection of B. abortus DNA. M: 1 kb Ladder, Lane 1: Negative control (physiological serum), Lane 2: Positive control (B. abortus ATTCC 23455), 3 and 4: Samples with Wright titer ≥1/20, 5 and 6: samples with no Wright titer.
Fig. 3.
Fig. 3.
The PCR assay for the detection of B. melitensis DNA. M: 1 kb Ladder, Lane 1: Negative control (physiological serum), Lane 2: Positive control (B. melitensis ATCC 23457), 3 and 4: Samples with Wright titer ≥1/20, 5 and 6: samples with no Wright titer.
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References

    1. Budak F, Göral G, Heper Y, Yilmaz E, Aymak F, Baştürk B, et al. IL-10 and IL-6 gene polymorphisms as potential host susceptibility factors in Brucellosis. Cytokine 2007;38:32–36. - PubMed
    1. Yu WL, Nielsen K. Review of detection of Brucella spp. by polymerase chain reaction. Croat Med J 2010;51:306–313. - PMC - PubMed
    1. Smith JA. Brucella Lipopolysaccharide and pathogenicity: The core of the matter. Virulence 2018;9:379–382. - PMC - PubMed
    1. Refai M. Incidence and control of brucellosis in the Near East region. Vet Microbiol 2002;90:81–110. - PubMed
    1. Hasanjani Roushan MR, Mohrez M, Smailnejad Gangi SM, Soleimani Amiri MJ, Hajiahmadi M. Epidemiological features and clinical manifestations in 469 adult patients with brucellosis in Babol, Northern Iran. Epidemiol Infect 2004;132:1109–1114. - PMC - PubMed

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