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. 2017 Jul 5;7(13):e2364.
doi: 10.21769/BioProtoc.2364.

Tumorigenicity Assay in Nude Mice

Feng Du  1 , Xiaodi Zhao  1 , Daiming Fan  1
Affiliations

Tumorigenicity Assay in Nude Mice

Feng Du et al. Bio Protoc. .

Abstract

Tumorigenicity refers to the ability of cultured cells to develop viable tumors in immune-deficient animals. The goal of this protocol is to illustrate tumorigenicity assay by subcutaneous tumor-cell-transplantation in nude mice. Target cells are transplanted to 6-week-old nude mice subcutaneously and the tumor growth is monitored over a period of observation or treatment. When tumor grows to a pre-determined size or by the end of the limited period, the nude mice will be euthanatized and the xenograft will be removed for further examination.

Keywords: Nude mice; Tumor cell transplantation; Tumor xenograft model; Tumorigenicity.

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Figures

Figure 1.
Figure 1.. Disinfection of the nude mice dorsal skin.
The nude mice were restrained by grasping the skin over the shoulders with left thumb and index finger. Disinfect the dorsal skin gently with 70%-ethanol-saturated cotton balls around the injection site for 2-3 times.
Figure 2.
Figure 2.. Subcutaneous injection of cell suspension into the nude mice.
A. Inserting the needle into the skin on the dorsal flank to reach the subcutaneous pocket but not the underlying muscle layer; B. Expelling the contents of the syringe for the subcutaneous implant.
See this image and copyright information in PMC

References

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