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. 2022:2363:1-12.
doi: 10.1007/978-1-0716-1653-6_1.

Purification of Leaf Mitochondria from Arabidopsis thaliana Using Percoll Density Gradients

Huy Cuong Tran  1 , Olivier Van Aken  2
Affiliations

Purification of Leaf Mitochondria from Arabidopsis thaliana Using Percoll Density Gradients

Huy Cuong Tran et al. Methods Mol Biol. 2022.

Abstract

The study of plant mitochondria often requires isolation of mitochondria from plant tissues in intact and functional form. Here, we describe an effective procedure of mitochondrial isolation from leaf tissues and whole seedlings of the model dicot species Arabidopsis thaliana by using differential centrifugation and continuous Percoll density gradients.

Keywords: Arabidopsis; Density gradients; Mitochondria; Mitochondrial isolation.

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