Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Jan;36(1):e14490.
doi: 10.1111/ctr.14490. Epub 2021 Oct 1.

Longitudinal profiling of plasma and urine metabolites during liver regeneration in living liver donors

Teodoro Bottiglieri  1 , Xuan Wang  2 , Erland Arning  1 , Hoylan Fernandez  3 , Anji Wall  3 , Greg McKenna  3 , Richard Ruiz  3 , Nicholas Onaca  3 , James Trotter  4 , Michael Lawrence  5 , Bashoo Naziruddin  5 , Sumeet K Asrani  4 , Giuliano Testa  3
Affiliations

Longitudinal profiling of plasma and urine metabolites during liver regeneration in living liver donors

Teodoro Bottiglieri et al. Clin Transplant. 2022 Jan.

Abstract

Background: Knowledge of metabolic processes affected by major hepatectomy (MHx), and the metabolic pathways involved in liver regeneration and recovery of function, is limited and mainly derived from animal models. Assessment of restoration of hepatic function is essential in human living liver donors (LD).

Methods: We used a targeted metabolomic approach to longitudinally quantify changes in plasma and urine biomarkers from healthy LD. The biomarkers were analyzed before MHx and at scheduled intervals up to 12 months thereafter.

Results: Marked changes were found in the concentration of 15 primary and secondary plasma bile acids. Most significant changes occurred 2 days after MHx and persisted for up to 3 months. In addition, there were significant changes in acylcarnitine, phospholipid, and amino acid metabolism. The sum of aromatic amino acids and the Fischer ratio, both metabolic markers of liver damage, and the symmetrically demethylated arginine to arginine ratio, a marker of kidney function, were affected.

Conclusions: This is the first comprehensive longitudinal study investigating metabolic processes during recovery of liver function after MHx in LD. It provides further evidence of full restoration of metabolic processes 3 months after MHx and supports future investigation to understand how metabolic changes affect donors' hepatic function.

Trial registration: ClinicalTrials.gov NCT01922323.

Keywords: P180 metabolites; bile acids; liver regeneration; living liver donation; metabolic indicators; metabolomics; methylation metabolites.

PubMed Disclaimer

References

REFERENCES

    1. Park GC, Song GW, Moon DB, Lee SG. A review of current status of living donor liver transplantation. Hepatobiliary Surg Nutr. 2016;5(2):107-117.
    1. Ibis C, Asenov Y, Akin M, Azamat IF, Sivrikoz N, Gurtekin B. Factors affecting liver regeneration in living donors after hepatectomy. Med Sci Monit. 2017;23:5986-5993.
    1. Choi SH, Kwon JH, Kim KW, et al. Measurement of liver volumes by portal vein flow by Doppler ultrasound in living donor liver transplantation. Clin Transplant. 2017;31(9):e13050.
    1. Tejura TK, Pita A, Romero C, Genyk YS, Sher L, Palmer SL. Living donor liver transplantation: post-operative imaging follow-up of right lobe liver donors. Abdom Radiol (NY). 2018;43(10):2673-2678.
    1. Jochum C, Beste M, Penndorf V, et al. Quantitative liver function tests in donors and recipients of living donor liver transplantation. Liver Transpl. 2006;12(4):544-549.

Associated data

LinkOut - more resources