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. 2021 Nov;135(6):2263-2274.
doi: 10.1007/s00414-021-02695-9. Epub 2021 Sep 22.

Development and validation of novel 8-dye short tandem repeat multiplex system for forensic applications

Bowei Jiang  1 , Weifeng Qu  2 , Feng Wang  1 , Liming Zhang  1 , Haibo Rong  1 , Jienan Li  2 , Dan Wen  2 , Moutanou Modeste Judes Zeye  2 , Wei He  2 , Chudong Wang  2 , Hao Xing  2 , Tao Zhang  1 , Chuan Jin  1 , Li Chen  1 , Ying Liu  2 , Jifeng Cai  2 , Lagabaiyila Zha  3
Affiliations

Development and validation of novel 8-dye short tandem repeat multiplex system for forensic applications

Bowei Jiang et al. Int J Legal Med. 2021 Nov.

Abstract

DNA profiling of short tandem repeats (STRs) is the primary method for genotyping forensic samples. However, degraded DNA and trace samples are still major problems for commercial 5- or 6-dye STR kits. In order to improve the performance of this method, we developed a novel 8-dye STR multiplex system containing 18 autosomal loci (D3S1358, D1S1656, TPOX, D16S539, vWA, D6S1043, D2S1338, CSF1PO, D19S433, D7S820, FGA, D8S1179, D5S818, D13S317, TH01, D21S11, D12S391, and PentaD) and the sex-determining locus Amelogenin, with all fragments smaller than 330 bases. Validation was carried out as recommended by the Scientific Working Group on DNA Analysis Methods. The results showed that complete profiles were obtainable when the input DNA was as low as 0.0625 ng. Full profiles were obtained even in the presence of inhibitors such as humic acid (< 300 ng/μl), hematin (< 100 μM), and indigo (0.01%). The 8-dye STR multiplex system also showed good performance in the detection degraded DNA samples. These results indicate that the 8-dye STR multiplex system is suitable for human DNA genotyping, including for difficult forensic materials.

Keywords: Developmental validation; GA118-24B genetic analyzer; STR; 8-dye STR multiplex system.

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