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. 2022 May 30;74(10):1850-1854.
doi: 10.1093/cid/ciab848.

Accuracy of a Rapid Multiplex Polymerase Chain Reaction Plus a Chromogenic Phenotypic Test Algorithm for Detection of Extended-Spectrum β-Lactamase and Carbapenemase-Producing Gram-Negative Bacilli in Positive Blood Culture Bottles

Sean Wei Xiang Ong  1   2 , Pei Yun Hon  1 , Sharon Syn Hui Wee  3 , Jonathan Wei Zhong Chia  4 , Shehara Mendis  4 , Ezlyn Izharuddin  1 , Ray Junhao Lin  1   2 , Po Ying Chia  1   2   5 , Rees Chin Swee Sim  4 , Mark I-Cheng Chen  1   6 , Angela Chow  5   6   7 , Joanne Yoong  6 , David Chien Lye  1   2   5   8 , Christine B Teng  9 , Paul Anantharajah Tambyah  8   10 , Ritu Banerjee  11 , Robin Patel  12 , Partha Pratim De  4 , Shawn Vasoo  1   2   5
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Accuracy of a Rapid Multiplex Polymerase Chain Reaction Plus a Chromogenic Phenotypic Test Algorithm for Detection of Extended-Spectrum β-Lactamase and Carbapenemase-Producing Gram-Negative Bacilli in Positive Blood Culture Bottles

Sean Wei Xiang Ong et al. Clin Infect Dis. .

Abstract

We studied the performance of an algorithm combining multiplex polymerase chain reaction with phenotypic detection of extended-spectrum β-lactamases and carbapenemases directly from positive blood culture bottles in patients with gram-negative bacteremia and found good concordance with routine cultures. Such an algorithm may be a tool to improve time to optimal therapy in patients with gram-negative bacteremia.

Trial registration: ClinicalTrials.gov NCT02743585.

Keywords: ESBL; antimicrobial resistance; carbapenemase; diagnostics; gram-negative bacteria; rapid test kits.

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