Molecular characterization of a new mitovirus hosted by the ectomycorrhizal fungus Albatrellopsis flettii
- PMID: 34554305
- DOI: 10.1007/s00705-021-05250-4
Molecular characterization of a new mitovirus hosted by the ectomycorrhizal fungus Albatrellopsis flettii
Abstract
The complete genome of a novel mycovirus, Albatrellopsis flettii mitovirus 1 (AfMV1), hosted by the basidiomycetous ectomycorrhizal fungus Albatrellopsis flettii (Morse ex Pouzar) Audet, was sequenced and analyzed. The full-length cDNA sequence, obtained from a dsRNA replication intermediate of the AfMV1 genome, is 3037 bp in length with a predicted G+C content of 40.66%. Sequence analysis revealed that a single large open reading frame (ORF) is present on the positive strand when the mold mitochondrial genetic code is applied. The single ORF encodes a putative RNA-dependent RNA polymerase of 859 amino acids with a predicted molecular weight of 97.05 kDa that shares the closest similarity with the corresponding protein of Entomophthora muscae mitovirus 7, with 43.38% sequence identity. Phylogenetic analysis showed that AfMV1 could be classified as a new member of the genus Mitovirus within the family Mitoviridae. This is the first report of the complete genome sequence of a new mitovirus, AfMV1, isolated from the basidiomycetous ectomycorrhizal fungus A. flettii.
© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.
References
-
- Giehl RF, von Wiren N (2014) Root nutrient foraging. Plant Physiol 166(2):509–517. https://doi.org/10.1104/pp.114.245225 - DOI - PubMed - PMC
-
- Brundrett MC, Tedersoo L (2018) Evolutionary history of mycorrhizal symbioses and global host plant diversity. New Phytol 220(4):1108–1115. https://doi.org/10.1111/nph.14976 - DOI - PubMed
-
- Smith S, Read D (2008) Mycorrhizal symbiosis, 3rd, edition. Academic Press
-
- Tripathi S, Mishra SK, Varma A (2017) Mycorrhizal Fungi as Control Agents Against Plant Pathogens. In: Varma A. PR, Tuteja N. (ed) Mycorrhiza - Nutrient Uptake, Biocontrol, Ecorestoration. Springer, Cham. doi: https://doi.org/10.1007/978-3-319-68867-1_8
-
- Garg N, Singh S, Kashyap L (2017) Arbuscular Mycorrhizal Fungi and Heavy Metal Tolerance in Plants: An Insight into Physiological and Molecular Mechanisms. In: Varma A. PR, Tuteja N. (ed) Mycorrhiza - Nutrient Uptake, Biocontrol, Ecorestoration. Springer, Cham. doi: https://doi.org/10.1007/978-3-319-68867-1_4
MeSH terms
Substances
LinkOut - more resources
Full Text Sources