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. 2022:2389:177-199.
doi: 10.1007/978-1-0716-1783-0_15.

Generating Cerebral Organoids from Human Pluripotent Stem Cells

Leon Chew #  1 , Adam Añonuevo #  1 , Erin Knock  2
Affiliations

Generating Cerebral Organoids from Human Pluripotent Stem Cells

Leon Chew et al. Methods Mol Biol. 2022.

Abstract

3D brain organoids derived from human pluripotent stem cells (hPSCs) possess the remarkable ability to self-organize and differentiate into tissue resembling the early human fetal brain. Brain organoids provide a powerful tool for studying human brain development and disease in an in vitro system. Here we describe a protocol for the differentiation of hPSCs to human cerebral organoids using a commercially available kit (STEMdiff™ Cerebral Organoid Kit) and discuss methods to scale up the protocol in a high-throughput manner.

Keywords: Brain; In vitro; Neural differentiation; Neurodevelopment; Organoid; Stem cells; Three-dimensional.

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