Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021 Oct;11(10):424.
doi: 10.1007/s13205-021-02967-6. Epub 2021 Sep 3.

Thrips diversity of cotton ecosystem and the role of parthenium pollen grains in the transmission of tobacco streak virus (TSV) infection in cotton

S Rageshwari  1   2 , S Vinodkumar  1   2 , P Renukadevi  3 , V G Malathi  4 , S Nakkeeran  2
Affiliations

Thrips diversity of cotton ecosystem and the role of parthenium pollen grains in the transmission of tobacco streak virus (TSV) infection in cotton

S Rageshwari et al. 3 Biotech. 2021 Oct.

Abstract

Survey in the cotton fields of Coimbatore and Erode districts confirmed the presence of three different thrips species including, Scirtothrips dorsalis, Scirtothrips oligochaetus, and Frankliniella occidentalis. Their identity was confirmed through morphometric analysis and molecular characterization. Tobacco streak virus (TSV) was detected in the leaves and pollen grains of both parthenium and cotton plants collected from infected cotton fields. The presence of TSV was confirmed through immuno-detection by direct antigen coating enzyme-linked immuno sorbent assay (DAC-ELISA). Further confirmation was accomplished by reverse transcriptase polymerase chain reaction (RT-PCR) using TSV coat protein-specific primers. Other than parthenium, weed plants in the cotton field such as Trianthema portulacastrum, Boerhavia diffusa, and Amaranthus sp. were also confirmed for TSV infection through RT-PCR. Parthenium hysterophorus plants acts as a silent carrier of TSV and they occasionally produced symptoms. Among all the randomly collected thrips samples, two pooled samples were detected positive for TSV through RT-PCR.

Supplementary information: The online version contains supplementary material available at 10.1007/s13205-021-02967-6.

Keywords: Parthenium pollen grains; TSV transmission in cotton; Thrips dynamics; Tobacco streak virus.

PubMed Disclaimer

Conflict of interest statement

Conflict of interestAs corresponding author, I confirm that there is no conflict of interest to disclose. The manuscript was reviewed by all the authors before submission.

Figures

Fig. 1
Fig. 1
Morphological characters of Scirtothrips dorsalis and Frankliniella occidentalis used for identification (Antenna segments, abdominal region). Images were captured at 40X Magnification in LEICA MZ16 stereo zoom microscope with a scale of 100 μm
Fig. 2
Fig. 2
Phylogenetic tree generated from aligned nucleotide sequences of mitochondrial cytochrome oxidase subunit I gene of study isolate and other thrips species. The tree was generated using neighbor joining method with 1000 bootstrap replications and cut off value of 70% in MEGA 7.0
Fig. 3
Fig. 3
Natural infection on weeds of cotton ecosystem exhibiting symptoms of TSV
Fig. 4
Fig. 4
Microscopic observation of parthenium pollen grain in the necrotic zone of symptomatic leaves. A Pollen grains at 40X magnification. B Pollen grains in the necrotic area at 10X magnification
See this image and copyright information in PMC

References

    1. Asokan R, Kumar NK, Kumar V, Ranganath H. Molecular differences in the mitochondrial cytochrome oxidase I (mtCOI) gene and development of a species-specific marker for onion thrips, Thrips tabaci Lindeman, and melon thrips, T. palmi Karny (Thysanoptera: Thripidae), vectors of tospoviruses (Bunyaviridae) Bull Entomol Res. 2007;97(5):461. doi: 10.1017/S0007485307005147. - DOI - PubMed
    1. Brunner P, Fleming C, Frey J. A molecular identification key for economically important thrips species (Thysanoptera: Thripidae) using direct sequencing and a PCR-RFLP-based approach. Agric for Entomol. 2002;4(2):127–136. doi: 10.1046/j.1461-9563.2002.00132.x. - DOI
    1. Chomczynski P, Sacchi N. The single-step method of RNA isolation by acid guanidinium thiocyanate–phenol–chloroform extraction: twenty-something years on. Nat Protoc. 2006;1(2):581–585. doi: 10.1038/nprot.2006.83. - DOI - PubMed
    1. Costa AS, Lima NVDC (1976) Transmissão do vírus da necrose branca do fumo por Frankliniella sp. In: Resumos, IX Congresso Brasileiro de Fitopatologia. Campinas, p 68
    1. Cranston PS, Gullan PJ. Encyclopedia of insects. London: Elsevier; 2009. Phylogeny of insects; pp. 780–793.

LinkOut - more resources