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. 2021 Sep 27;11(10):jkab244.
doi: 10.1093/g3journal/jkab244.

Chromosome-scale assembly of wild barley accession "OUH602"

Kazuhiro Sato  1 , Martin Mascher  2   3 , Axel Himmelbach  2 , Georg Haberer  4 , Manuel Spannagl  4 , Nils Stein  2   5
Affiliations

Chromosome-scale assembly of wild barley accession "OUH602"

Kazuhiro Sato et al. G3 (Bethesda). .

Abstract

Barley (Hordeum vulgare) was domesticated from its wild ancestral form ca. 10,000 years ago in the Fertile Crescent and is widely cultivated throughout the world, except for in tropical areas. The genome size of both cultivated barley and its conspecific wild ancestor is approximately 5 Gb. High-quality chromosome-level assemblies of 19 cultivated and one wild barley genotype were recently established by pan-genome analysis. Here, we release another equivalent short-read assembly of the wild barley accession "OUH602." A series of genetic and genomic resources were developed for this genotype in prior studies. Our assembly contains more than 4.4 Gb of sequence, with a scaffold N50 value of over 10 Mb. The haplotype shows high collinearity with the most recently updated barley reference genome, "Morex" V3, with some inversions. Gene projections based on "Morex" gene models revealed 46,807 protein-coding sequences and 43,375 protein-coding genes. Alignments to publicly available sequences of bacterial artificial chromosome (BAC) clones of "OUH602" confirm the high accuracy of the assembly. Since more loci of interest have been identified in "OUH602," the release of this assembly, with detailed genomic information, should accelerate gene identification and the utilization of this key wild barley accession.

Keywords: Hordeum vulgare ssp. spontaneum; OUH602; genome assembly; pseudomolecules; wild barley.

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Figures

Figure 1
Figure 1
Intra-chromosomal Hi-C contact matrices. Gray lines mark contig boundaries. Some centromeres are spanned by a single scaffold. The absence of off-(anti)-diagonal signals supports the accuracy of the assembly.
Figure 2
Figure 2
Alignment of pseudomolecules of “OUH602” to “Morex” V3 by a single chromosome. Areas between dotted red lines show inversions.
See this image and copyright information in PMC

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