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Comment
. 2021 Oct;36(5):1232-1235.
doi: 10.1007/s12250-021-00452-1. Epub 2021 Sep 27.

The Infection and Pathogenicity of SARS-CoV-2 Variant B.1.351 in hACE2 Mice

Qi Chen #  1 , Xing-Yao Huang #  1 , Ying Tian #  1   2 , Changfa Fan #  3 , Mengxu Sun #  1 , Chao Zhou  1 , Ruiting Li  1 , Rong-Rong Zhang  1 , Guizhen Wu  4 , Cheng-Feng Qin  5
Affiliations
Comment

The Infection and Pathogenicity of SARS-CoV-2 Variant B.1.351 in hACE2 Mice

Qi Chen et al. Virol Sin. 2021 Oct.
No abstract available

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Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
Infection of SARS-CoV-2 variant B.1.351 in hACE2 mice. A 12-month-old female hACE2 mice were intranasally infected with 1.2 × 104 pfu of SARS-CoV-2 variant B.1.351 or PBS (mock), and sacrificed on 1, 3, 5 and 7 dpi for tissue collection (n = 3). B Body weight changes of hACE2 mice intranasally infected with B.1.351 or PBS (mock) (n = 8). C Tissue distribution of SARS-CoV-2 sgRNA. Each tissue or serum was processed and viral sgRNA copies were analyzed by real-time qPCR. Dotted lines denote the detection limit. D ISH assay for SARS-CoV-2 RNA in lung tissues. Positive signals are shown in brown. E Immunostaining of lung tissues with a SARS-CoV-2 N protein-specific mAb. F Hematoxylin and eosin (H&E) staining analysis showing alveolar septal thickening, alveolar damage, denatured bronchiolar epithelial cells (black arrow), hemorrhage (yellow arrow), and macrophages infiltration (green arrow) in infected mice. G Semiquantitative analysis of the H&E-stained lung sections (n = 3). H IHC staining analysis for neutrophils (Neu+) and T cells (CD3+) in hACE2 mice on 3 days post infection. I Relative cytokine production in serum of infected hACE2 mice on 3 days post infection. For a more detailed description of methods used, please see the online Supplementary materials.
See this image and copyright information in PMC

Comment on

  • Detection of a SARS-CoV-2 variant of concern in South Africa.
    Tegally H, Wilkinson E, Giovanetti M, Iranzadeh A, Fonseca V, Giandhari J, Doolabh D, Pillay S, San EJ, Msomi N, Mlisana K, von Gottberg A, Walaza S, Allam M, Ismail A, Mohale T, Glass AJ, Engelbrecht S, Van Zyl G, Preiser W, Petruccione F, Sigal A, Hardie D, Marais G, Hsiao NY, Korsman S, Davies MA, Tyers L, Mudau I, York D, Maslo C, Goedhals D, Abrahams S, Laguda-Akingba O, Alisoltani-Dehkordi A, Godzik A, Wibmer CK, Sewell BT, Lourenço J, Alcantara LCJ, Kosakovsky Pond SL, Weaver S, Martin D, Lessells RJ, Bhiman JN, Williamson C, de Oliveira T. Tegally H, et al. Nature. 2021 Apr;592(7854):438-443. doi: 10.1038/s41586-021-03402-9. Epub 2021 Mar 9. Nature. 2021. PMID: 33690265

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Supplementary concepts