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. 2021 Oct;69(10):2844-2845.
doi: 10.4103/ijo.IJO_1912_21.

Innovative intra-operative rapid detection test for visualisation of mucor

Prithvi Chandrakanth  1 , Prasanna Venkatesh Ramesh  2 , Trichy Narayanan Janakiram  3 , Shruthy Vaishali Ramesh  4 , K Aji  5 , K S Chandrakanth  6 , Meena K Ramesh  7 , Ramesh Rajasekaran  8
Affiliations

Innovative intra-operative rapid detection test for visualisation of mucor

Prithvi Chandrakanth et al. Indian J Ophthalmol. 2021 Oct.

Abstract

In this manuscript, we report a rapid intra-operative detection test for visualisation of mucor, with the use of the Smartphone-based intraocular lens microscope (IOLSCOPE). IOLSCOPE helps not only in detecting tissue affected by mucor, but also makes sure that the surgeon does not leave any fungus behind; similar to the principles employed by a 'Frozen section' in conventional general surgery. In the technique proposed by us, we have used an undigested tissue sample, to quickly diagnose mucor, intra-operatively. Thus, in view of tackling the COVID-19 and mucor duplet, IOLSCOPE can be considered by ophthalmologists and otorhinolaryngologists for its rapid screening property, convenience of ease, good quality images and cost effectiveness.

Keywords: COVID-19; IOLSCOPE; Mucor; ROCM; Rapid detection test.

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Conflict of interest statement

None

Figures

Figure 1
Figure 1
(a) T2-weighted MRI image showing orbital soft tissue involvement (red arrow) in the left side with maxillary sinus and anterior ethmoidal sinus involvement in both sides with mucormycosis (green arrows). (b) T2-weighted MRI image showing involvement of left ethmoidal sinuses and both maxillary sinuses with mucormycosis (green arrows). (c) Image of a 45-year-old male patient presenting in the post COVID-19 recovery period with left eye ptosis, proptosis, and chemosis with restriction of extraocular movements
Figure 2
Figure 2
(a) Image showing functional endoscopic sinus surgery done for debriding the necrosed mucosa along with the infected tissue. (b) Immediate analysis of the debrided tissue under the IOLSCOPE in the operating room
Figure 3
Figure 3
(a) Image of debrided tissue under the IOLSCOPE on a potassium hydroxide (KOH) mount showing filamentous fungi (red arrow) adjacent to a black mass of undigested tissue. (b) Image of the same tissue under light microscope showing filamentous fungi (red arrow) adjacent to a black mass of debrided tissue
See this image and copyright information in PMC

References

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