Enhanced Antibacterial Potential of Amoxicillin against Helicobacter pylori Mediated by Lactobionic Acid Coated Zn-MOFs

Affiliations

¹ Institute of Chemical Sciences, University of Peshawar, Peshawar 25120, Pakistan.
² H.E.J. Research Institute of Chemistry, International Centre for Chemical and Biological Sciences, University of Karachi, Karachi 74200, Pakistan.
³ Department of Biological Sciences, National University of Medical Sciences, Rawalpindi 46000, Pakistan.
⁴ Department of Pharmacy, University of Malakand, Chakdara 18800, Pakistan.
⁵ Department of Chemistry, College of Sciences, University of Ha'il, Ha'il 2440, Saudi Arabia.
⁶ Microbiology and Immunology Unit, Department of Pathology, College of Medicine, Jouf University, Sakaka 72341, Saudi Arabia.
⁷ Department of Medical Microbiology and Immunology, College of Medicine, Menoufia University, Menoufia 32511, Egypt.
⁸ Department of Pharmacology and Therapeutics, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, Egypt.
⁹ Department of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia.

PMID: 34572653
PMCID: PMC8470299
DOI: 10.3390/antibiotics10091071

Enhanced Antibacterial Potential of Amoxicillin against Helicobacter pylori Mediated by Lactobionic Acid Coated Zn-MOFs

Haseena et al. Antibiotics (Basel). 2021.

. 2021 Sep 4;10(9):1071.

doi: 10.3390/antibiotics10091071.

Authors

Affiliations

¹ Institute of Chemical Sciences, University of Peshawar, Peshawar 25120, Pakistan.
² H.E.J. Research Institute of Chemistry, International Centre for Chemical and Biological Sciences, University of Karachi, Karachi 74200, Pakistan.
³ Department of Biological Sciences, National University of Medical Sciences, Rawalpindi 46000, Pakistan.
⁴ Department of Pharmacy, University of Malakand, Chakdara 18800, Pakistan.
⁵ Department of Chemistry, College of Sciences, University of Ha'il, Ha'il 2440, Saudi Arabia.
⁶ Microbiology and Immunology Unit, Department of Pathology, College of Medicine, Jouf University, Sakaka 72341, Saudi Arabia.
⁷ Department of Medical Microbiology and Immunology, College of Medicine, Menoufia University, Menoufia 32511, Egypt.
⁸ Department of Pharmacology and Therapeutics, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, Egypt.
⁹ Department of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia.

PMID: 34572653
PMCID: PMC8470299
DOI: 10.3390/antibiotics10091071

Abstract

H. pylori (Helicobacter pylori) causes a common chronic infectious disease and infects around 4.4 billion people worldwide. H. pylori was classified as a member of the primary class of stomach cancer (stomach adenocarcinoma). Hence, this study was conducted to design a novel lactobionic acid (LBA)-coated Zn-MOFs to enhance bactericidal activity of Amoxicillin (AMX) against H. pylori. The synthesized Zn-MOFs were characterized by various techniques which included Dynamic Light Scattering (DLS), Fourier Transform Infrared (FT-IR) Spectroscopy, Powder X-ray diffraction, scanning electron microscope, and atomic force microscope. They were capable of encapsulating an increased amount of AMX and investigated for their efficacy to enhance the antibacterial potential of their loaded drug candidate. Interestingly, it was found that LBA-coated Zn-MOFs significantly reduced the IC₅₀, MIC, and MBIC values of AMX against H. pylori. Morphological investigation of treated bacterial cells further authenticated the above results as LBA-coated Zn-MOFs-treated cells underwent complete distortion compared with non-coated AMX loaded Zn-MOFs. Based on the results of the study, it can be suggested that LBA-coated Zn-MOFs may be an effective alternate candidate to provide new perspective for the treatment of H. pylori infections.

Keywords: Amoxicillin; H. pylori; lactobionic acid; metal organic frameworks (MOFs).

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

**Figure 1**
FTIR Spectrum of ATA, Zn-Acetate, Zn-MOFs, AMX, Am-ZnMOFs, and L-Am-Zn-MOFs.

**Figure 2**
The UV spectrum of pure AMX showing the peak at 230 nm (a). UV spectrum of Zn-MOFs showing the peak at 326 nm (b). UV spectrum of AMX-loaded Zn-MOFs showed a slight variation in AMX peak (c). UV spectrum of AMX after its release from Zn-MOFs (d).

**Figure 3**
Representative AFM images showing size variation between Zn-MOFs (a) and LBA-coated Am-Zn-MOFs (b).

**Figure 4**
Powder X-ray diffraction pattern of Zn-MOFs.

**Figure 5**
AFM images of *H. pylori* control cells (a), *H. pylori* AMX treated (b), *H. pylori* Zn-MOF treated (c), *H. pylori* Am-Zn-MOF treated (d), and *H. pylori* L-Am-Zn-MOF treated (e).

**Figure 6**
*H. pylori* control cells (a), *H. pylori* AMX treated (b), *H. pylori* Zn-MOF treated (c), *H. pylori* Am-Zn-MOF treated (d), and *H. pylori* L-Am-Zn-MOF treated (e).

See this image and copyright information in PMC

Cited by

Improving curcumin bactericidal potential against multi-drug resistant bacteria via its loading in polydopamine coated zinc-based metal-organic frameworks.
Jabbar A, Rehman K, Jabri T, Kanwal T, Perveen S, Rashid MA, Kazi M, Ahmad Khan S, Saifullah S, Shah MR. Jabbar A, et al. Drug Deliv. 2023 Dec;30(1):2159587. doi: 10.1080/10717544.2022.2159587. Drug Deliv. 2023. PMID: 36718806 Free PMC article.
Application of Metal-Organic Framework in Diagnosis and Treatment of Diabetes.
Gao Q, Bai Q, Zheng C, Sun N, Liu J, Chen W, Hu F, Lu T. Gao Q, et al. Biomolecules. 2022 Sep 5;12(9):1240. doi: 10.3390/biom12091240. Biomolecules. 2022. PMID: 36139080 Free PMC article. Review.
Hesperidin-, Curcumin-, and Amphotericin B- Based Nano-Formulations as Potential Antibacterials.
Akbar N, Kawish M, Khan NA, Shah MR, Alharbi AM, Alfahemi H, Siddiqui R. Akbar N, et al. Antibiotics (Basel). 2022 May 20;11(5):696. doi: 10.3390/antibiotics11050696. Antibiotics (Basel). 2022. PMID: 35625340 Free PMC article.
Recent advances in metal-organic frameworks for antibacterial applications: mechanisms and emerging strategies.
Tian Y, Wang B, Zhang Z, Kai T, Wu P, Ding P. Tian Y, et al. RSC Adv. 2025 Jul 28;15(33):26710-26727. doi: 10.1039/d5ra02955d. eCollection 2025 Jul 25. RSC Adv. 2025. PMID: 40727299 Free PMC article. Review.
Nanoparticle formulations for therapeutic delivery, pathogen imaging and theranostic applications in bacterial infections.
Jiang L, Ding L, Liu G. Jiang L, et al. Theranostics. 2023 Mar 5;13(5):1545-1570. doi: 10.7150/thno.82790. eCollection 2023. Theranostics. 2023. PMID: 37056563 Free PMC article. Review.

See all "Cited by" articles

References

1. Wilson W. Prevention of infective endocarditis: Guidelines from the American heart association: A guideline from the American heart association rheumatic fever, endocarditis, and Kawasaki disease committee, council on cardiovascular disease in the young, and the council on clinical cardiology, council on cardiovascular surgery and anesthesia, and the quality of care and outcomes research interdisciplinary working group. Circulation. 2007;116:1736–1754. - PubMed
1. Akhavan B.J., Khanna N.R., Vijhani P. Amoxicillin. StatPearls Publishing; San Francisco, CA, USA: 2020. - PubMed
1. Drissi M. Antibiotic susceptibility and mechanisms of β-lactam resistance among clinical strains of Pseudomonas aeruginosa: First report in Algeria. Med. Mal. Infect. 2008;38:187–191. doi: 10.1016/j.medmal.2008.01.009. - DOI - PubMed
1. Hooi J.K. Global prevalence of Helicobacter pylori infection: Systematic review and meta-analysis. J. Gastroenterol. 2017;153:420–429. doi: 10.1053/j.gastro.2017.04.022. - DOI - PubMed
1. Hatakeyama M., Brzozowski T. Pathogenesis of Helicobacter pylori infection. Helicobacter. 2006;11:14–20. doi: 10.1111/j.1478-405X.2006.00424.x. - DOI - PubMed

LinkOut - more resources

Full Text Sources

Save citation to file

Email citation

Add to Collections

Add to My Bibliography

Your saved search

Create a file for external citation management software

Your RSS Feed

Enhanced Antibacterial Potential of Amoxicillin against Helicobacter pylori Mediated by Lactobionic Acid Coated Zn-MOFs

Affiliations

Enhanced Antibacterial Potential of Amoxicillin against Helicobacter pylori Mediated by Lactobionic Acid Coated Zn-MOFs

Authors

Affiliations

Abstract

Conflict of interest statement

Figures

Similar articles

Cited by

References

LinkOut - more resources

Full Text Sources