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. 2021:1345:1-6.
doi: 10.1007/978-3-030-82735-9_1.

Characterization Methods of Acellularized Tissue and Organs

Layla Shojaie  1   2 , Yekta Rahimi  1 , Masoumeh Majidi Zolbin  1 , Faezeh Daghigh  3   4 , Abdol-Mohammad Kajbafzadeh  5   6
Affiliations

Characterization Methods of Acellularized Tissue and Organs

Layla Shojaie et al. Adv Exp Med Biol. 2021.

Abstract

The extracellular matrix (ECM) of mammalian organs and tissues has been applied as a substitute scaffold to simplify the restoration and reconstruction of several tissues. Such scaffolds are prepared in various arrangements including sheets, powders, and hydrogels. One of the more applicable processes is using natural scaffolds, for this purpose discarded tissues or organs are naturally derived by processes that comprised decellularization of following tissues or organs. Protection of the complex structure and 3D (three dimensional) ultrastructure of the ECM is extremely necessary but it is predictable that all protocols of decellularization end in disruption of the architecture and potential loss of surface organization and configuration. Tissue decellularization with conservation of ECM bioactivity and integrity can be improved by providing well-designed protocols regarding the agents and decellularization techniques operated during processing. An overview of the characterization of decellularized scaffolds and the role of reagnets can validate the applied methods' efficacy.

Keywords: Acellular; Characterize; Scaffold; Tissue engineering.

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