Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2021:1345:85-102.
doi: 10.1007/978-3-030-82735-9_8.

The Isolated Mucosa of the Rat Colon Decellularization, Microscopy and Cell Cultures

Pedro Mestres-Ventura  1 , Laura López Gómez  2 , Gilberto Del Rosario Hernández  3 , Teresa Núñez López  4
Affiliations

The Isolated Mucosa of the Rat Colon Decellularization, Microscopy and Cell Cultures

Pedro Mestres-Ventura et al. Adv Exp Med Biol. 2021.

Abstract

This project presents the most important findings of the studies, which we carried out in our laboratory on the decellularization of the rat isolated colonic mucosa. We have also included some details of the experiences gathered with the muscle layer as well as the whole wall of the colon. The question of the cytocompatibility of this new substrate has been addressed with the application of primary cultures of human cells and well-established cell lines. The possible applications in experimental and medical settings will be discussed.

Keywords: Cell cultures; Colon; Decellularization; ECM scaffold; Microscopy; Rat.

PubMed Disclaimer

References

    1. Andres H, Bock R, Bridges RJ, Rummel W, Schreiner J (1985) Submucosal plexus and electrolyte transport across rat colonic mucosa. J Physiol 364:301–312 - DOI
    1. Badylak SF (2007) The extracellular matrix as a biological scaffold material. Biomaterials 28(25):3587–3593. https://dio.org/10.1016/j.biomaterials.2007.04.043
    1. Badylak SF, Kropp B, McPherson T, Liang H, Snyder PW (1998) Small intestine submucosa: a rapid resorbed bioscaffold for augmentation cystoplasty. Tissue Eng 4(4):379–387 - DOI
    1. Badylak SF, Freytes DO, Gilbert TW (2009) Extracellular matrix as a biological scaffold material: structure and function. Acta Biomater 5:1–13 - DOI
    1. Barnes CA, Brison J, Michel R, Brown BN, Castner DG, Badylak SF, Ratner BD (2011) The surface molecular functionality of decellularized extracellular matrices. Biomaterials 32(1):137–143. https://doi.org/10.1016/j.biomaterials.2010.09.007 - DOI - PubMed

LinkOut - more resources