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. 1986 Apr;83(8):2571-5.
doi: 10.1073/pnas.83.8.2571.

Analysis of Agrobacterium tumefaciens virulence mutants in leaf discs

R B Horsch, H J Klee, S Stachel, S C Winans, E W Nester, S G Rogers, R T Fraley

Analysis of Agrobacterium tumefaciens virulence mutants in leaf discs

R B Horsch et al. Proc Natl Acad Sci U S A. 1986 Apr.

Abstract

The leaf disc transformation system provides a simple means to score expression of various T-DNA markers within days of infection by Agrobacterium tumefaciens as well as long-term selection for growth of transformed callus and shoots. In this report, we describe the application of this system to evaluation of marker transfer and integration in a comprehensive set of defined avirulent mutants of A. tumefaciens. We conclude that virC is not essential when the T-DNA is present on a binary vector. The most likely explanation for this is that the virC gene product is involved in generation of a T-DNA intermediate.

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