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. 2021 Sep 28;188(10):355.
doi: 10.1007/s00604-021-05001-x.

Label-free and sensitive MiRNA detection based on turn-on fluorescence of DNA-templated silver nanoclusters coupled with duplex-specific nuclease-assisted signal amplification

Gui-Min Ma  1 , Li-Wei Huo  1 , Yin-Xia Tong  1 , Yu-Cong Wang  1 , Cui-Ping Li  2 , Hong-Xia Jia  3
Affiliations

Label-free and sensitive MiRNA detection based on turn-on fluorescence of DNA-templated silver nanoclusters coupled with duplex-specific nuclease-assisted signal amplification

Gui-Min Ma et al. Mikrochim Acta. .

Abstract

A novel strategy for microRNAs (miRNAs) detection has been developed utilizing duplex-specific nuclease-assisted signal amplification (DSNSA) and guanine-rich DNA-enhanced fluorescence of DNA-templated silver nanoclusters (AgNCs). The combination between target miRNA, DSNSA, and AgNCs is achieved by the unique design of DNA sequences. Target miRNA opens the hairpin structure of the Hairpin DNA probe (HP) by hybridizing with the HP and initiates the duplex-specific nuclease-assisted signal amplification (DSNSA) reaction. The DSNSA reaction generates the release of the guanine-rich DNA sequence, which can turn on the fluorescence of the dark AgNCs by hybridizing with the DNA template of the dark AgNCs. The fluorescence intensity of AgNCs corresponds to the dosage of the target miRNA. This is measured at 630 nm by exciting at 560 nm. The constructed method exhibits a low detection limit (~8.3 fmol), a great dynamic range of more than three orders of magnitude, and excellent selectivity. Moreover, it has a good performance for miR-21 detection in complex biological samples. A novel strategy for microRNAs (miRNAs) detection has been developed utilizing duplex-specific nuclease-assisted signal amplification (DSNSA) and guanine-rich DNA-enhanced fluorescence of DNA-templated silver nanoclusters (AgNCs).

Keywords: DNA template; Duplex-specific nuclease-assisted signal amplification; Fluorescence enhancement; MicroRNAs; Silver nanoclusters.

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References

    1. Petty JT, Zheng J, Hud NV, Dickson RM (2004)DNA-templated Ag nanocluster formation. J Am Chem Soc 126:5207–5212. https://doi.org/10.1021/ja031931o - DOI - PubMed
    1. Su YT, Lan GY, Chen WY, Chang HT (2010) Detection of copper ions through recovery of the fluorescence of DNA-templatedcopper/silver nanoclusters in the presence of mercaptopropionic acid. Anal Chem 82:8566–8572. https://doi.org/10.1021/ac101659d - DOI - PubMed
    1. Guo W, Yuan J, Wang E (2009)Oligonucleotide-stabilized Ag nanoclusters as novel fluorescence probes for the highly selective and sensitive detection of the Hg2+ ion. Chem Commun 23:3395–3397. https://doi.org/10.1039/b821518a - DOI
    1. Shen C, Xia X, Hu S, Yang M, Wang J (2015) Silver nanoclusters-based fluorescence assay of protein kinase activity and inhibition. Anal Chem 87:693–698. https://doi.org/10.1021/ac503492k - DOI - PubMed
    1. Han B, Wang E (2011)Oligonucleotide-stabilized fluorescent silver nanoclusters for sensitive detection of biothiols in biological fluids. Biosens Bioelectron 26:2585–2589. https://doi.org/10.1016/j.bios.2010.11.011 - DOI - PubMed

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