Preparation of Centella asiatica loaded gelatin/chitosan/nonwoven fabric composite hydrogel wound dressing with antibacterial property

Abstract

Antibiotics abuse and the emergence of massive drug-resistant bacteria have become the major obstacles in the medical system. Thus, designing an antibiotic-free wound dressing with antibacterial activity and decent biocompatibility is urgently desired. Herein, the sandwich-like composite hydrogel wound dressings were developed by intercalating nonwoven fabrics (NF) as the middle layer, gelatin and chitosan (Gel-CS) hydrogel loaded with Centella asiatica (CA) as the base materials. In addition, soaking strategy was employed to improve the mechanical properties of hydrogels. The hydrogels exhibited uniform microporous structure, stable mechanical property, high water absorbency, as well as water vapor transmission rate. After loading with CA, the composite wound dressing showed the sustained drug release properties in vitro and excellent antibacterial activity against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli). The cytotoxicity results demonstrated that the composite hydrogels had good biocompatibility. This work indicates that the nonwoven composite hydrogels have broad application prospects in the field of medical care in the future.

Keywords: Antibacterial activity; Centella asiatica; Hydrogel; Nonwoven fabric; Wound dressing.