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. 2021 Sep 30;18(2):627-675.
doi: 10.1515/ijb-2020-0043. eCollection 2022 Nov 1.

Spike detection for calcium activity

Hermine Biermé  1 , Camille Constant  1 , Anne Duittoz  2 , Christine Georgelin  3
Affiliations

Spike detection for calcium activity

Hermine Biermé et al. Int J Biostat. .

Abstract

We present in this paper a global methodology for the spike detection in a biological context of fluorescence recording of GnRH-neurons calcium activity. For this purpose we first propose a simple stochastic model that could mimic experimental time series by considering an autoregressive AR(1) process with a linear trend and specific innovations involving spiking times. Estimators of parameters with asymptotic normality are established and used to set up a statistical test on estimated innovations in order to detect spikes. We compare several procedures and illustrate on biological data the performance of our procedure.

Keywords: 62F03; 62F12; 62M10; Secondary: 92B25; autoregressive process; neuronal activity; peaks detection; time series.

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References

    1. Levine, JE, Pau, KY, Ramirez, VD, Jackson, GL. Simultaneous measurement of luteinizing hormone-releasing hormone and luteinizing hormone release in unanesthetized, ovariectomized sheep. Endocrinology 1982;111:1449–55. https://doi.org/10.1210/endo-111-5-1449.
    1. Clarke, IJ, Cummins, JT. The temporal relationship between gonadotropin releasing hormone (GnRH) and luteinizing (LH) secretion in ovariectomized ewes. Endocrinology 1982;111:1737–9. https://doi.org/10.1210/endo-111-5-1737.
    1. Caraty, A, Orgeur, P, Thiery, J-C. Demonstration of the pulsatile secretion of LH-RH into hypophysial portal blood of ewes using an original technic for multiple samples. Comptes rendus des séances de l’Académie des sciences. Série III, Sciences de la vie 1982;295:10.
    1. Moenter, SM. GnRH neuron electrophysiology: a decade of study. Brain Res 2010;1364:10–24. https://doi.org/10.1016/j.brainres.2010.09.066.
    1. Wray, S. From nose to brain: development of gonadotrophin-releasing hormone-1 neurones. J Neuroendocrinol 2010;22:743–53. https://doi.org/10.1111/j.1365-2826.2010.02034.x.

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