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. 2021 Dec;44(12):2679-2696.
doi: 10.1007/s00449-021-02637-0. Epub 2021 Oct 1.

Biogenic synthesis and characterization of selenium nanoparticles and their applications with special reference to antibacterial, antioxidant, anticancer and photocatalytic activity

Shraddha Pandey  1 , Nikee Awasthee  2 , Anusmita Shekher  2 , Lal Chand Rai  1 , Subash Chandra Gupta  2 , Santosh Kumar Dubey  3
Affiliations

Biogenic synthesis and characterization of selenium nanoparticles and their applications with special reference to antibacterial, antioxidant, anticancer and photocatalytic activity

Shraddha Pandey et al. Bioprocess Biosyst Eng. 2021 Dec.

Abstract

Oxyanions of selenium, selenite (SeO3)2- and selenate (SeO4)2- are toxic to terrestrial and aquatic biota but few microorganisms including cyanobacteria are resistant to high levels of selenite. Cyanobacteria evade selenite toxicity through bioreduction and synthesis of selenium nanoparticles (SeNPs). In this study, extracellular biosynthesis of SeNPs (Se0) using cyanobacterium, Anabaena sp. PCC 7120 on exposure to sodium selenite and characterization was done by using UV-visible spectroscopy, SEM-EDX, TEM and FTIR analyses which confirmed spherical shape with size range of 5-50 nm diameter. These biogenic SeNPs demonstrated significant antibacterial and anti-biofilm activity against bacterial pathogens. Furthermore, these SeNPs showed high antioxidant activity at minimum concentration of 50 µg/mL and significant anti-proliferative activity against HeLa cell line with IC50 value of 5.5 µg/mL. The SeNPs also induced accumulation of cancer cells in the sub-G1 phase which was clearly observed in cellular and nuclear morphology. These biofabricated SeNPs also reduced and decolorized toxic methylene blue dye significantly through photocatalytic degradation. Therefore Anabaena sp. PCC 7120 may be employed as a green bioresource to synthesize SeNPs with potential applications in medicine and environmental bioremediation.

Keywords: Anti-proliferative; Antioxidant; Bioreduction; SeNPs; Selenium.

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