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. 2022 Jan;8(1):237-244.
doi: 10.1002/vms3.648. Epub 2021 Oct 2.

Antimicrobial resistance profiling and burden of resistance genes in zoonotic Salmonella isolated from broiler chicken

Tridip Das  1   2 , Eaftekhar Ahmed Rana  1 , Avijit Dutta  1 , Md Bayazid Bostami  3 , Mizanur Rahman  3 , Probir Deb  1 , Chandan Nath  1 , Himel Barua  1 , Paritosh Kumar Biswas  1   2
Affiliations

Antimicrobial resistance profiling and burden of resistance genes in zoonotic Salmonella isolated from broiler chicken

Tridip Das et al. Vet Med Sci. 2022 Jan.

Abstract

Background: Salmonella is frequently found in poultry of which only motile serovars have zoonotic significance due to their potential to induce human gastrointestinal infections. Antimicrobial resistance, being a public health concern, the emergence of multidrug-resistant (MDR) Salmonella serotypes affecting food chain has greater impact worldwide.

Aim: Information on circulation of zoonotic Salmonella strains in commercial poultry farm level is limited in many parts of the world. This cross-sectional study was aimed to investigate the zoonotic Salmonella strains circulating in the broiler farm environment with their detailed antimicrobial resistance profiling.

Methods: Pooled faecal samples were collected randomly from commercial broiler farms of Chattogram district, Bangladesh. Standard bacteriological procedure was followed to isolate Salmonella, and identification was confirmed by genus specific polymerase chain reaction (PCR). After phenotypic characterisation of resistance profile against eight antimicrobials by disc diffusion technique, all strains were screened by PCR for some selected resistance genes. RESULTS: Out of the 350 samples, Salmonella was isolated and identified from 86 samples. In antimicrobial sensitivity testing, more than 98.8% isolates showed resistance to ampicillin and 94.2% to tetracycline followed by enrofloxacin (56%) and ciprofloxacin (50%). Notably, 94% isolates were found to be MDR. The results of PCR assays revealed that 81.4% of the isolates were carrying the tetA gene, 19.8% the tetB and 10.47% the tetC gene. The prevalence of the isolates bearing the blaTEM , blaCTX-M and Sul-I gene were 95.4%, 7.0 % and 37.2 %, respectively.

Conclusion: There is a great risk to secure healthy poultry products due to the circulation of these MDR zoonotic Salmonella.

Keywords: Salmonella; antimicrobial resistance; chicken; resistance genes.

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Conflict of interest statement

The authors have no potential conflict of interest.

Figures

FIGURE 1
FIGURE 1
Geographical distribution of Salmonella positive and negative broiler farms, where each red‐ or blue coloured circle indicates single broiler farm
FIGURE 2
FIGURE 2
Distribution of antimicrobial resistance phenotype and genotype of Salmonella isolates, where TET = tetracycline, GEN = gentamicin, CIP = ciprofloxacin, ENR = enrofloxacin, SXT = sulfamethoxazole/trimethoprim, AMP = ampicillin, CRO = ceftriaxone, FOX = cefoxitin
FIGURE 3
FIGURE 3
Multidrug‐resistance profile of all Salmonella isolates [n = 86], where red bars indicate multidrug‐resistant (MDR) isolates and green bars indicate other than MDR isolates
See this image and copyright information in PMC

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