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Review
. 2021;69(10):970-975.
doi: 10.1248/cpb.c21-00306.

Emerging Separation Techniques in Supercritical Fluid Chromatography

Kazuhiro Yamamoto  1 , Koichi Machida  1 , Akira Kotani  1 , Hideki Hakamata  1
Affiliations
Review

Emerging Separation Techniques in Supercritical Fluid Chromatography

Kazuhiro Yamamoto et al. Chem Pharm Bull (Tokyo). 2021.

Abstract

Supercritical fluid chromatography (SFC) has unique separative characteristics distinguished from those of HPLC and gas chromatography. At present, SFC is widely used and there are many applications in various biological, medical, and pharmaceutical fields. In this review, we focus on recently developed novel techniques related to SFC separation including: new column stationary phases, microfluidics, two-dimensional separation, and gas-liquid separation. In addition, we discuss the application of SFC using a water-containing modifier to biological molecules such as amino acids, peptides, and small proteins that had been challenging analytes.

Keywords: biological molecule; separation technique; supercritical fluid chromatography.

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