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Review
. 2021 Sep 14;6(38):24297-24303.
doi: 10.1021/acsomega.1c02563. eCollection 2021 Sep 28.

Microfluidics-Integrated Sensors toward Rapid Detection of Single Nucleotide Variations

Marziyeh Hajialyani  1 , Leila Hosseinzadeh  2 , Jie Jayne Wu  1
Affiliations
Review

Microfluidics-Integrated Sensors toward Rapid Detection of Single Nucleotide Variations

Marziyeh Hajialyani et al. ACS Omega. .

Abstract

Rapid detection of single nucleotide variations (SNVs) is of critical importance to early diagnosis of several diseases and the prediction of diverse responses to a specific treatment. Based on the information published in the literature, discrimination of SNVs is a developing area of study with great research enthusiasm and is also an area that can benefit from microfluidics-integrated designs. This review provides a brief overview of different microfluidics-based strategies for rapid detection of SNVs and mismatched bases. Sensors based on various microfluidic formats, such as paper-based microfluidic biosensors, droplet-based microfluidic systems, and magnetic bead-based microfluidic biosensors, have been discussed with respect to their specific pros and cons for SNV detection. These systems have shown promise for distributed on-site diagnostics in personalized medicine.

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Conflict of interest statement

The authors declare no competing financial interest.

Figures

Figure 1
Figure 1
Components of a lateral flow biosensor for rapid detection of SNVs. Reproduced with permission from ref (5). Copyright 2018 Elsevier.
Figure 2
Figure 2
Photograph and schematic of a droplet-based microfluidic biosensor used by Cui et al. for SNP detection. Reproduced with permission from ref (20). Copyright 2017 Elsevier.
See this image and copyright information in PMC

References

    1. Chen S.; Shamsi M. H. Biosensors-on-chip: A topical review. J. Micromech. Microeng. 2017, 27 (8), 083001.10.1088/1361-6439/aa7117. - DOI
    1. Ma L.-H.; Wang H.-B.; Zhang T.; Xuan Y.; Li C.; Chen W.; Liu B.; Zhao Y.-D. Visual simultaneous detection of single nucleotide polymorphism of tumor susceptibility gene and marker alpha-fetoprotein based on double-labeled colloidal gold probe with lateral flow strip biosensor. Sens. Actuators, B 2019, 298, 126819.10.1016/j.snb.2019.126819. - DOI
    1. Liu G.; Lao R.; Xu L.; Xu Q.; Li L.; Zhang M.; Song S.; Fan C. Single-nucleotide polymorphism genotyping using a novel multiplexed electrochemical biosensor with nonfouling surface. Biosens. Bioelectron. 2013, 42, 516–521. 10.1016/j.bios.2012.11.005. - DOI - PubMed
    1. Ilkhani H.; Zhang H.; Zhou A. A novel three-dimensional microTAS chip for ultra-selective single base mismatched Cryptosporidium DNA biosensor. Sens. Actuators, B 2019, 282, 675–683. 10.1016/j.snb.2018.11.120. - DOI
    1. Lin C.-W.; Kuo J.-C.; Liu H.-L.; Cheng Y.-S.; Huang H.-L. A new method for detection of single nucleotide polymorphism in a female reproduction-associated gene, tmigd1, of Anas platyrhynchos using a strip biosensor with gold nanoparticles. Poult. Sci. 2018, 97 (10), 3456–3462. 10.3382/ps/pey240. - DOI - PubMed