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. 2022 Feb;49(2):302-310.
doi: 10.1111/1440-1681.13600. Epub 2021 Oct 21.

Transcription factor EB enhances autophagy and ameliorates palmitate-induced insulin resistance at least partly via upregulating AMPK activity in skeletal muscle cells

Ping Wang  1 , Chun Guang Li  2 , Xian Zhou  2 , Shuzhe Ding  3   4
Affiliations

Transcription factor EB enhances autophagy and ameliorates palmitate-induced insulin resistance at least partly via upregulating AMPK activity in skeletal muscle cells

Ping Wang et al. Clin Exp Pharmacol Physiol. 2022 Feb.

Abstract

This study aimed to elucidate the role of transcription factor EB (TFEB) in protecting C2C12 myotubes against palmitate (PA)-induced insulin resistance (IR) and explored its mechanism associated with autophagy. PA treatment significantly decreased insulin sensitivity in myotubes and downregulated TFEB protein expression. TFEB overexpression significantly reversed the PA-suppressed glucose transporter 4 (GLUT4) protein expression and improved intracellular glucose uptake and consumption, and also alleviated the decrease of autophagy markers induced by PA. The effect of TFEB overexpression on GLUT4 was also abolished by the autophagy inhibitor 3-MA. In addition, AMPKɑ2-DN inhibited or abolished the effects of TFEB overexpression on upregulation of GLUT4 and PA-induced decrease of autophagy marker expressions. Taken together, our data demonstrated that upregulation of TFEB improved PA-induced IR in C2C12 myotubes by enhancing autophagy and upregulating AMPK activity. TFEB, as a critical regulator of glucose homeostasis in skeletal muscle cells, may be a potential therapeutic target for IR and Type 2 diabetes.

Keywords: AMPK; C2C12 myotubes; TFEB; autophagy; insulin resistance.

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References

REFERENCES

    1. Li H, Liu S, Yuan H, et al. Sestrin 2 induces autophagy and attenuates insulin resistance by regulating AMPK signaling in C2C12 myotubes. Exp Cell Res. 2017;354(1):18-24.
    1. Julibert A, Bibiloni M-D-M, Gallardo-alfaro L, et al. Metabolic syndrome features and excess weight were inversely associated with nut consumption after 1-year follow-up in the PREDIMED-plus study. J Nutr. 2020;150(12):3161-3170.
    1. Tchetina E-V, Markova G-A, Sharapova E-P. Insulin resistance in osteoarthritis: similar mechanisms to type 2 diabetes mellitus. J Nutr Metab. 2020;5(21):4143802.
    1. Yang L, Qiu T, Yao X, et al. Taurine protects against arsenic trioxide-induced insulin resistance via ROS-Autophagy pathway in skeletal muscle. Int J Biochem Cell Biol. 2019;7(112):50-60.
    1. Periasamy M, Herrera J-L, Reis F-C-G. Skeletal muscle thermogenesis and its role in whole body energy metabolism. Diabetes Metab J. 2017;41(5):327-336.

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