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. 2021 Oct 7;10(40):e0074621.
doi: 10.1128/MRA.00746-21. Epub 2021 Oct 7.

Evaluation of Genome Sequences of the Bacteriophages JeTaime and Luna22

Maximiliano F Flota #  1 , Caitlyn R Moss #  1 , Mitchell F Balish  2 , Nikki Chen  1 , Kristen C Cherry  1 , Kathleen A Cornely  3 , Hayley M D'Alessandro  1 , Mouna S DiBenedetto  1 , Joseph A DeGiorgis  3 , Steven Grant Dixon  1 , Emily G Dombrowski  1 , Megan K Edwards  1 , Jonathan C Eskew-Martin  1 , Isabel E P Finnegan  1 , Abanob G Hanna  1 , Sarah E Hunter  1 , Sabrina L Johnson  1 , Virginia A L Kenan  1 , Chanelle Kendrick  3 , Lucas C Licaj  1 , Victoria C Maldonado  1 , Megan G Mazzei  1 , Gracen E Mitrick  1 , Bradford L J Nelson  1 , Jui S Patel  1 , Alexander I Parry  1 , Kacey M Smekrud  1 , Kirsten K Snyder  1 , Jonathan A Stewart  1 , Fredrick K Swiger  1 , Madison K Thomas  1 , Josiah C Waters  1 , Christine A Byrum  1
Affiliations

Evaluation of Genome Sequences of the Bacteriophages JeTaime and Luna22

Maximiliano F Flota et al. Microbiol Resour Announc. .

Abstract

The mycobacteriophages JeTaime (E cluster) and Luna22 (Q cluster) were isolated from soil in Providence, Rhode Island, and Charleston, South Carolina, respectively, using a Mycobacterium smegmatis mc2 155 host. The genome of JeTaime is 75,099 bp (142 predicted genes), and that of Luna22 is 53,730 bp (87 predicted genes). Both phages exhibit Siphoviridae morphology.

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Figures

FIG 1
FIG 1
JeTaime (A) and Luna22 (B) morphology, examined using transmission electron microscopy. High-titer lysates placed on Formvar-coated grids were negatively stained with 1% uranyl acetate (2). JeTaime was imaged using a JEOL 20× transmission electron microscope (100 kV), and Luna22 was imaged using a JEOL 1010 transmission electron microscope (80 kV). Bars, 50 nm.
See this image and copyright information in PMC

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