Effect of TiF4/NaF and chitosan solutions on biofilm formation and prevention of dentin demineralization

Abstract

Objective: This study evaluated the effect of experimental solutions containing TiF₄/NaF and chitosan on bacterial species of microcosm biofilm and on dentin demineralization.

Design: Microcosm biofilm was produced from human saliva mixed with McBain medium (0.2% sucrose) on bovine dentin for 5 days, under 5% CO₂ and 37 °C. From the 2nd day to 5th day, the treatments were applied (1×60s/day) as following: (1) NaF (500 ppm F^-, positive control); (2) TiF₄ and NaF (TiF₄: 190 ppm Ti⁴⁺ and 300 ppm F^-; NaF: 190 ppm F^-); (3) similar to 2 plus 0.5% chitosan (Ch 500 mPa.s, 75% deacetylation); (4) phosphate buffer solution (negative control); and (5) 0.5% chitosan (Ch 500 mPa.s, 75% deacetylation). CFU counting was performed for total microorganism, total streptococci, total lactobacilli and mutans streptococci. Dentin demineralization was measured by transverse microradiography-TMR. The data were compared using ANOVA/Tukey or Kruskal-Wallis/Dunn tests (p < 0.05).

Results: No differences were found between the treatments with respect to CFU counting (p > 0.05). Dentin treated with TiF₄/NaF plus chitosan solution presented the lowest demineralization compared to the negative control and pure chitosan solution. On the other hand, this experimental solution did not significantly differ from TiF₄/NaF solution, being both able to significantly reduce mineral loss.

Conclusion: TiF₄/NaF plus chitosan solution, at suitable pH to be clinically applicable, had no antimicrobial effect, but it was able to reduce dentin caries development under this model.

Keywords: Chitosan; Dental caries; Fluoride; Oral biofilm.