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. 2021 Sep 2:44:100936.
doi: 10.1016/j.nmni.2021.100936. eCollection 2021 Nov.

Analysing the HERV-K env, np9, rec and gag expression in cervical tissues

S Tavakolian  1 , H Goudarzi  1 , A Moridi  2 , E Faghihloo  1
Affiliations

Analysing the HERV-K env, np9, rec and gag expression in cervical tissues

S Tavakolian et al. New Microbes New Infect. .

Abstract

Cervical cancer is considered to be the fourth common cancer. It is assumed that numerous risk factors, especially infectious ones, can have a detrimental effect on cervical cancer. In this study, we evaluated the expression of Herv-K env, np9, rec and gag in cervical tissues. After RNA extraction and cDNA sensitizing of 12 cervical cancer tissues and CIN3, 51 CIN1,2 and 18 normal ones, Herv-K env, np9, rec and gag were assessed using quantitative real-time PCR analysis. There was a decrease in the level of HERV-K env expression in cervical cancer and CIN 1-3 in compression with normal tissues. Cervical cancer and CIN3 indicated the most increase in expression. Meanwhile, we observed an increase in gag and rec expression in CIN 1,2; although cervical cancer and CIN 3 had a decrease in rec and gag expression, we did not report any changes in np expression. In conclusion, given the relationship between HERV-associated genes and cervical cancer, our study suggests that these genes can be useful for cancer diagnosis. However, further investigations are needed to provide a better perspective about the effectiveness of these genes in the diagnostic strategies of gastrointestinal cancer. These results are just an observation that could open a wider investigation to test the correlation between the expression of these genes and cervical cancer.

Keywords: Cervical cancer; HERV-K env; gag; np9; rec.

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Figures

Fig. 1
Fig. 1
Decrease in env mRNA expression in cervical cancer and CIN 1-3.
Fig. 2
Fig. 2
gag expression in cervical tissues.
Fig. 3
Fig. 3
rec expression in cervical tissues.
Fig. 4
Fig. 4
No changes of np expression in cervical tissues.
See this image and copyright information in PMC

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