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. 2021 Sep 21:8:731067.
doi: 10.3389/fmed.2021.731067. eCollection 2021.

Reliability of the Multiplex CytoBead CeliAK Immunoassay to Assess Anti-tTG IgA for Celiac Disease Screening

Diyora Abdukhakimova  1 , Kuanysh Dossybayeva  1 , Anna Grechka  2 , Zhaina Almukhamedova  2 , Alyona Boltanova  3 , Larissa Kozina  3 , Kadisha Nurgaliyeva  4 , Liliya Hasanova  2 , Matthew N Tanko  1   4 , Dimitri Poddighe  1   2
Affiliations

Reliability of the Multiplex CytoBead CeliAK Immunoassay to Assess Anti-tTG IgA for Celiac Disease Screening

Diyora Abdukhakimova et al. Front Med (Lausanne). .

Abstract

Background and Objective: The diagnosis of Celiac Disease (CD) is first based on the positivity for specific serological markers. The CytoBead CeliAK immunoassay simultaneously measures antibodies (IgA) directed to tissue transglutaminase (tTG), endomysium (EMA), and deamidated gliadin (DG), in addition to providing a control for total IgA levels. The aim of this study is to assess the reliability of this multiplex assay to detect anti-tTG IgA positive patients, compared with a conventional single-parameter enzyme-linked immunosorbent assay (ELISA). Methods: Serum samples from 149 pediatric patients were assessed by both CytoBead CeliAK immunoassay and ELISA, in order to evaluate their concordance for the measurement of anti-tTG IgA. Results: The measurement of anti-tTG IgA by CytoBead CeliAK immunoassay basically showed a complete concordance rate with the conventional and single-parameter ELISA, according to the respective cutoff values (3 U/ml and 10 U/ml). Conclusions: Our comparative analysis demonstrates a substantial equivalency between multiplex CytoBead CeliAK assay and the single-parameter conventional ELISA to assess anti-tTG IgA antibody in the context of the screening for CD in children. Importantly, CytoBead CeliAK assay could present some preanalytic, analytic, and economic advantages.

Keywords: ELISA; anti-tissue transglutaminase antibody; celiac disease; children; immunoglobulin A; multiplex assay; screening.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. Moreover, the funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.

Figures

Figure 1
Figure 1
Plot correlating anti-tTG IgA levels by CytoBead CeliAK immunoassay and anti-tTG IgA levels by ELISA (*this black square corresponds to two patients with identical values; N, total number of patients included in this blue quadrant; red dashed line, respective cutoff values for each assay).
See this image and copyright information in PMC

References

    1. Lindfors K, Ciacci C, Kurppa K, Lundin KEA, Makharia GK, Mearin ML, et al. . Coeliac disease. Nat Rev Dis Primers. (2019) 5:3. 10.1038/s41572-018-0054-z - DOI - PubMed
    1. Kelly D, Mearin ML, Ribes Koninckx C. Pediatric celiac disease: earlier diagnosis for better lifelong health. J Pediatr Gastroenterol Nutr. (2018) 67:e106. 10.1097/MPG.0000000000002105 - DOI - PubMed
    1. Poddighe D, Capittini C, Gaviglio I, Brambilla I, Marseglia GL. HLA-DQB1*02 allele in children with celiac disease: potential usefulness for screening strategies. Int J Immunogenet. (2019) 46:342–5. 10.1111/iji.12441 - DOI - PubMed
    1. Ludvigsson JF, Murray JA. Epidemiology of celiac disease. Gastroenterol Clin North Am. (2019) 48:1–18. 10.1016/j.gtc.2018.09.004 - DOI - PubMed
    1. Stahl MG, Geno Rasmussen C, Dong F, Waugh K, Norris JM, Baxter J, et al. . Mass screening for celiac disease: the autoimmunity screening for kids study. Am J Gastroenterol. (2021) 116:180–7. 10.14309/ajg.0000000000000751 - DOI - PMC - PubMed