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. 2022:2303:63-70.
doi: 10.1007/978-1-0716-1398-6_6.

A Nonradioactive Method to Measure Hyaluronan Synthase Activity

Davide Vigetti  1 , Evgenia Karousou  1 , Manuela Viola  1 , Alberto Passi  2
Affiliations

A Nonradioactive Method to Measure Hyaluronan Synthase Activity

Davide Vigetti et al. Methods Mol Biol. 2022.

Abstract

Hyaluronan (HA) is a component of the extracellular matrix that is involved in many physiological and pathological processes. As HA modulates several functions (i.e., cell proliferation and migration, inflammation), its presence in the tissues can have positive or negative effects. HA synthases (HAS) are a family of three isoenzymes located on the plasma membrane that are responsible for the production of such polysaccharide and, therefore, their activity is critical to determine the accumulation of HA in tissues. Here, we describe a nonradioactive method to quantify the HAS enzymatic activity in crude cellular membrane preparation.

Keywords: Enzymatic activity; Glycosaminoglycan synthesis; Hyaluronan; Hyaluronan synthase; UDP-sugars.

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