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. 2022 Feb;85(2):728-737.
doi: 10.1002/jemt.23944. Epub 2021 Oct 10.

An AFM study of the nanostructural response of New Zealand white rabbit Achilles tendons to cyclic loading

Anas K Al Makhzoomi  1 , Thomas B Kirk  2 , Garry T Allison  3
Affiliations

An AFM study of the nanostructural response of New Zealand white rabbit Achilles tendons to cyclic loading

Anas K Al Makhzoomi et al. Microsc Res Tech. 2022 Feb.

Abstract

The nanostructural response of New Zealand white rabbit Achilles tendons to a fatigue damage model was assessed quantitatively and qualitatively using the endpoint of dose assessments of each tendon from our previous study. The change in mechanical properties was assessed concurrently with nanostructural change in the same non-viable intact tendon. Atomic force microscopy was used to study the elongation of D-periodicities, and the changes were compared both within the same fibril bundle and between fibril bundles. D-periodicities increased due to both increased strain and increasing numbers of fatigue cycles. Although no significant difference in D-periodicity lengthening was found between fibril bundles, the lengthening of D-periodicity correlated strongly with the overall tendon mechanical changes. The accurate quantification of fibril elongation in response to macroscopic applied strain assisted in assessing the complex structure-function relationship in Achilles tendons.

Keywords: D-periodicity; atomic force microscopy; concurrent nanostructural and mechanical assessment; tendon structure-function relationships.

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References

REFERENCES

    1. Al Makhzoomi, A. K., Kirk, B. T., & Allison, G. T. (2021). A multiscale study of morphological changes in tendons following repeated cyclic loading. Manuscript submitted for publication.
    1. Al Makhzoomi, A. K., Kirk, T. B., Dye, D. E., & Allison, G. T. (2021). The influence of glycosaminoglycan proteoglycan side chains on tensile force transmission and the nanostructural properties of Achilles tendons. Microscopy Research and Technique, 1-11. https://doi.org/10.1002/jemt.23899
    1. Andarawis-Puri, N., & Flatow, E. L. (2011). Tendon fatigue in response to mechanical loading. Journal of Musculoskeletal and Neuronal Interactions, 11(2), 106-114.
    1. Archambault, J. (2003). Tendon micromechanics and research methods in tendinopathy. Journal of Musculoskeletal and Neuronal Interactions, 3(4), 326-328.
    1. Archambault, J. M., & Banes, A. J. (2005). Research methodology and animal modeling in tendinopathy. In N. Maffulli, P. Renström, & W. B. Leadbetter (Eds.), Tendon injuries: Basic science and clinical medicine (pp. 279-286). London, England: Springer.

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