Synthesis and use of galactolipid sulfotransferase substrate-analogue affinity probes: possible localization of the testicular enzyme

Abstract

Galactosyl ceramide (GC) is a substrate in vitro for galactolipid sulfotransferase preparations from brain, kidney, and testis. Biotinylated and light-sensitive azido derivatives of lysogalactosyl ceramide have been synthesized. These derivatives remain effective substrates for the testicular enzyme. Biotinylated GC has been used to localize specific GC-binding sites in frozen sections of rat testis. The binding pattern is consistent with the expected location of the testicular galactolipid sulfotransferase.