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. 1986 Aug;5(8):1967-71.
doi: 10.1002/j.1460-2075.1986.tb04451.x.

High affinity binding site for nuclear factor I next to the hepatitis B virus S gene promoter

Y Shaul, R Ben-Levy, T De-Medina

High affinity binding site for nuclear factor I next to the hepatitis B virus S gene promoter

Y Shaul et al. EMBO J. 1986 Aug.

Abstract

The hepatitis B virus (HBV) surface antigen (HBsAG) is encoded by the S gene under the regulation of a promoter in the pre-S1 region. The S gene promoter does not contain a 'TATA' box-like sequence, but there is a sequence resembling, in part, the late promoter of Simian virus 40 (SV40). In an attempt to study the regulation of the S gene promoter we looked for cellular proteins which bind to this region. We report here that a nuclear protein is tightly bound to the HBV genome at a position approximately 190 bases upstream from the S gene promoter. Evidence is provided to show that (a) this nuclear protein is the nuclear factor I (NF-I) that was previously found to be bound to the inverted terminal repeat of the adenovirus (Ad) DNA and to enhance Ad DNA replication in vitro and (b) this NF-I binding site is required for optimal activity of the S gene promoter.

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