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. 2021 Dec;43(12):3911-3921.
doi: 10.1002/hed.26888. Epub 2021 Oct 12.

miR-29a-3p promotes migration and invasion in ameloblastoma via Wnt/β-catenin signaling by targeting catenin beta interacting protein 1

Sai Liu  1 , Dongjuan Liu  1 , Jinwen Liu  1 , Jiayi Liu  1 , Ming Zhong  1   2
Affiliations

miR-29a-3p promotes migration and invasion in ameloblastoma via Wnt/β-catenin signaling by targeting catenin beta interacting protein 1

Sai Liu et al. Head Neck. 2021 Dec.

Abstract

Background: Ameloblastoma (AB) is a common epithelial odontogenic tumor. The Wnt/β-catenin pathway has been found to be related to AB invasion.

Methods: The alteration expression of microRNAs (miRNAs) and messenger RNAs (mRNAs) was performed by miRNA and mRNA microarray analysis and validated by quantitative real-time polymerase chain reaction (RT-PCR). The effects of miR-29a-3p on migration and invasion in AB cells were evaluated by a transwell assay. Bioinformatic prediction was conducted using the miRSystem and validated by quantitative RT-PCR, western blot, and a luciferase reporter assay.

Results: miR-29a-3p was overexpressed in AB tissues, which promoted the migration and invasion of AB cells in vitro. Catenin beta interacting protein 1 (CTNNBIP1), a negative regulator of the Wnt/β-catenin pathway, was predicted to be a target of miR-29a-3p. miR-29a-3p inhibited the expression of CTNNBIP1 and promoted the expression of the downstream molecules of the Wnt/β-catenin pathway.

Conclusions: miR-29a-3p promoted migration and invasion in AB via Wnt/β-catenin signaling by targeting CTNNBIP1.

Keywords: Wnt/β-catenin signaling; ameloblastoma; catenin beta interacting protein 1; invasion; miR-29a-3p.

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References

REFERENCES

    1. Effiom OA, Ogundana OM, Akinshipo AO, Akintoye SO. Ameloblastoma: current etiopathological concepts and management. Oral Dis. 2018;24(3):307-316. https://doi.org/10.1111/odi.12646
    1. Kreppel M, Zoller J. Ameloblastoma-clinical, radiological, and therapeutic findings. Oral Dis. 2018;24(1-2):63-66. https://doi.org/10.1111/odi.12702
    1. Nagatsuka H, Han PP, Tsujigiwa H, et al. Heparanase gene and protein expression in ameloblastoma: possible role in local invasion of tumor cells. Oral Oncol. 2005;41(5):542-548. https://doi.org/10.1016/j.oraloncology.2005.01.004
    1. Barberán-Soler S, Vo JM, Hogans RE, Dallas A, Johnston BH, Kazakov SA. Decreasing miRNA sequencing bias using a single adapter and circularization approach. Genome Biol. 2018;19(1):105. https://doi.org/10.1186/s13059-018-1488-z
    1. Rupaimoole R, Slack FJ. MicroRNA therapeutics: towards a new era for the management of cancer and other diseases. Nat Rev Drug Discov. 2017;16(3):203-222. https://doi.org/10.1038/nrd.2016.246

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