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. 1986 Nov 1;137(9):3002-5.

The assignment of chain specificities for anti-Ia monoclonal antibodies using L cell transfectants

D Landias, B N Beck, J M Buerstedde, S Degraw, D Klein, N Koch, D Murphy, M Pierres, T Tada, K Yamamoto, et al.
  • PMID: 3463623

The assignment of chain specificities for anti-Ia monoclonal antibodies using L cell transfectants

D Landias et al. J Immunol. .

Abstract

The chain specificities of 18 Ak and 26 Ab-reactive anti-Ia monoclonal antibodies have been determined. L cells were transfected with haplotype-matched (A alpha k:A beta k, A alpha b:A beta k) or haplotype-mismatched (A alpha k:A beta b, A alpha b:A beta k) cDNA pairs, lines expressing high levels of surface A complex were selected, and antibody reactivity with a panel of reagents was assessed by cytofluorimetric analysis. Most of the antibodies recognized a determinant specified by one chain, either alpha or (more commonly) beta. A few examples of more complex determinants were also observed. A knowledge of the chain specificities of anti-Ia monoclonal antibodies should prove useful for a variety of studies aimed at dissecting Ia structure-function relationships.

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