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Review
. 2022 Jul;65(7):1325-1341.
doi: 10.1007/s11427-021-1997-2. Epub 2021 Oct 9.

Techniques for increasing the yield of stem cell-derived exosomes: what factors may be involved?

Zi-Yuan Feng #  1 , Qing-Yi Zhang #  1 , Jie Tan  1 , Hui-Qi Xie  2
Affiliations
Review

Techniques for increasing the yield of stem cell-derived exosomes: what factors may be involved?

Zi-Yuan Feng et al. Sci China Life Sci. 2022 Jul.

Abstract

Exosomes are nano-scale extracellular vesicles secreted by cells and constitute an important part in the cell-cell communication. The main contents of the exosomes include proteins, microRNAs, and lipids. The mechanism and safety of stem cell-derived exosomes have rendered them a promising therapeutic strategy for regenerative medicine. Nevertheless, limited yield has restrained full explication of their functions and clinical applications To address this, various attempts have been made to explore the up- and down-stream manipulations in a bid to increase the production of exosomes. This review has recapitulated factors which may influence the yield of stem cell-derived exosomes, including selection and culture of stem cells, isolation and preservation of the exosomes, and development of artificial exosomes.

Keywords: exosome; stem cell; therapy; yield.

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Conflict of interest statement

Compliance and ethics The author(s) declare that they have no conflict of interest, and all authors have agreed with the publication of this article.

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