Characterization and function of circulating mucosal-associated invariant T cells and γδT cells in oral lichen planus

Abstract

Background: Oral lichen planus (OLP) is a T-cell-mediated chronic inflammatory disease with uncertain aetiology. Mucosal-associated invariant T (MAIT) cells and γδT cells are unconventional, innate-like T cells with immunoregulatory capacity. This study aimed to investigate the potential effects of MAIT and γδT cells on the pathogenesis of OLP.

Methods: Circulating MAIT cells and γδT cells were identified using flow cytometry. Surface proteins including CD4, CD8, CD69, CD103, CD49d, programmed death-1 (PD-1) and its ligand PD-L1 were assessed. Cytokines containing interleukin (IL)-4, IL-17, interferon (IFN)-γ, granzyme B and tumour necrosis factor (TNF)-α released by MAIT and γδT cells were measured following PMA and ionomycin stimulation.

Results: Circulating MAIT and γδT cells were deficient in OLP. The percentage of CD4⁺ , CD69⁺ , CD103⁺ and PD-1⁺ MAIT cells was increased in OLP, while that of CD8⁺ and CD49d⁺ MAIT cells was decreased. The percentage of CD103⁺ , PD-1⁺ and PD-L1⁺ γδT cells was upregulated in OLP. Both the MAIT and γδT cells in OLP produced less IL-4 than controls. The granzyme B-producing MAIT cells were increased, while γδT cells secreting granzyme B and TNF-α were reduced in OLP. IL-17 and IFN-γ in OLP MAIT and γδT cells were not significantly different from that in controls. The frequency of OLP MAIT cells and the MAIT/γδT rate were positively associated with the disease severity.

Conclusion: The deficient MAIT and γδT cells expressing functional proteins and releasing cytokines may play an immunoregulatory role in the pathogenesis of OLP.

Keywords: mucosal-associated invariant T cell; oral lichen planus; γδT cell.