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. 2021 Oct 5;14(19):5829.
doi: 10.3390/ma14195829.

Effect of Different Root Canal Irrigant Solutions on the Release of Dentin-Growth Factors: A Systematic Review and Meta-Analysis

Sandro Tavares  1 , Andrea Pintor  2 , Carlos Fernando de Almeida Barros Mourão  3 , Marcela Magno  2 , Pietro Montemezzi  3 , Roberto Sacco  1   4 , Gutemberg Alves  3 , Miriam Zaccaro Scelza  5
Affiliations

Effect of Different Root Canal Irrigant Solutions on the Release of Dentin-Growth Factors: A Systematic Review and Meta-Analysis

Sandro Tavares et al. Materials (Basel). .

Abstract

Irrigant solutions are used to promote dentin-growth factors (GF) release for regenerative endodontics. This review aimed to evaluate the reports comparing the release of GFs using different root canal irrigant solutions. Eligible studies compared the in vitro GF release in human teeth after the use of at least two distinct solutions. A search was conducted on Pubmed, Scopus, Web of Science, and Lilacs on 11 August 2021. Risk of bias was assessed using SciRAP. Study characteristics and quantitative data were extracted, and meta-analyses were performed for the mean difference (95% confidence interval) of the release of transforming growth factors Beta 1 (TGF-β1) by EDTA compared to other irrigants. Of sixteen eligible studies, eight were included in quantitative analysis. ELISA assays showed higher TGF-β1 release from 10% EDTA compared to 10% citric acid (p < 0.00001). Immunogold assays showed higher levels of TGF-β1 for 17% EDTA (p < 0.00001) compared to 10% citric acid. GRADE identified a low to very low certainty of evidence. These results point to an increased release of TGF-β1 in dentin treated with EDTA. The high heterogeneity and very low certainty of the evidence demand further studies before EDTA indication as a better irrigant for regenerative endodontics. Registration: CRD42020160871 (PROSPERO).

Keywords: dentin; growth factor; root canal irrigants.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
PRISMA flow diagram.
Figure 2
Figure 2
Forest plot for comparisons of TGF-β1 detected by ELISA method after exposure to 10% EDTA versus 10% citric acid.
Figure 3
Figure 3
Forest plot for comparisons of TGF-β1 detected by ELISA method after exposure to 17% EDTA versus other irrigants.
Figure 3
Figure 3
Forest plot for comparisons of TGF-β1 detected by ELISA method after exposure to 17% EDTA versus other irrigants.
Figure 4
Figure 4
Forest plot for comparisons of TGF-β1 detected by the immunogold method after exposure to EDTA versus 10% citric acid.
See this image and copyright information in PMC

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