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Review
. 2021 Dec;21(23-24):e2100145.
doi: 10.1002/pmic.202100145. Epub 2021 Oct 28.

Progress in methodologies and quality-control strategies in protein cross-linking mass spectrometry

Kumar Yugandhar  1   2 , Qiuye Zhao  1   2 , Shobhita Gupta  1   2 , Dapeng Xiong  1   2 , Haiyuan Yu  1   2
Affiliations
Review

Progress in methodologies and quality-control strategies in protein cross-linking mass spectrometry

Kumar Yugandhar et al. Proteomics. 2021 Dec.

Abstract

Deciphering the interaction networks and structural dynamics of proteins is pivotal to better understanding their biological functions. Cross-linking mass spectrometry (XL-MS) is a powerful and increasingly popular technology that provides information about protein-protein interactions and their structural constraints for individual proteins and multiprotein complexes on a proteome-scale. In this review, we first assess the coverage and depth of the XL-MS technique by utilizing publicly available datasets. We then delve into the progress in XL-MS experimental and computational methodologies and examine different quality-control strategies reported in the literature. Finally, we discuss the progress in XL-MS applications along with the scope for future improvements.

Keywords: cross-linking; interaction network; mass spectrometry; protein-protein; proteomics.

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References

REFERENCES

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