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. 2022 Jan;174(1):e13585.
doi: 10.1111/ppl.13585. Epub 2021 Oct 27.

Variety-specific transcript accumulation during reproductive stage in drought-stressed rice

Pratibha Gour  1 , Shivani Kansal  1 , Priyanka Agarwal  1 , Bhuwaneshwar Sharan Mishra  1 , Deepika Sharma  1 , Saloni Mathur  2 , Saurabh Raghuvanshi  1
Affiliations

Variety-specific transcript accumulation during reproductive stage in drought-stressed rice

Pratibha Gour et al. Physiol Plant. 2022 Jan.

Abstract

The divergence of natural stress tolerance mechanisms between species is an intriguing phenomenon. To study it in rice, a comparative transcriptome analysis was carried out in 'heading' stage tissue (flag leaf, panicles and roots) of Nagina 22 (N22; drought-tolerant) and IR64 (drought-sensitive) plants subjected to field drought. Interestingly, N22 showed almost double the number of differentially expressed genes (DEGs) than IR64. Many DEGs colocalized within drought-related QTLs responsible for grain yield and drought tolerance and also associated with drought tolerance and critical drought-related plant traits such as leaf rolling, trehalose content, sucrose and cellulose content. Besides, co-expression analysis of the DEGs revealed several 'hub' genes known to actively regulate drought stress response. Strikingly, 1366 DEGs, including 21 'hub' genes, showed a distinct opposite regulation in the two rice varieties under similar drought conditions. Annotation of these variety-specific DEGs (VS-DEGs) revealed that they are distributed in various biological pathways. Furthermore, 103 VS-DEGs were found to physically interact with over 1300 genes, including 32 that physically interact with other VS-DEGs as well. The promoter region of these genes has sequence variations among the two rice varieties, which might be in part responsible for their unique expression pattern.

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References

REFERENCES

    1. Bai, X., Huang, Y., Hu, Y., Liu, H., Zhang, B., Smaczniak, C. et al. (2017) Duplication of an upstream silencer of FZP increases grain yield in rice. Nature Plants, 3, 885-893.
    1. Baldoni, E., Bagnaresi, P., Locatelli, F., Mattana, M. & Genga, A. (2016) Comparative leaf and root transcriptomic analysis of two rice japonica cultivars reveals major differences in the root early response to osmotic stress. Rice, 9, 1-20.
    1. Balyan, S., Kumar, M., Mutum, R.D., Raghuvanshi, U., Agarwal, P., Mathur, S. et al. (2017) Identification of miRNA-mediated drought responsive multi-tiered regulatory network in drought tolerant rice, Nagina. Scientific Reports, 7, 1-17.
    1. Bernier, J., Atlin, G.N., Serraj, R., Kumar, A. & Spaner, D. (2008) Breeding upland rice for drought resistance. Journal of the Science of Food and Agriculture, 88, 927-939.
    1. Bohnert, H.J., Gong, Q., Li, P. & Ma, S. (2006) Unraveling abiotic stress tolerance mechanisms: getting genomics going. Current Opinion in Plant Biology, 9, 180-188.

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