doi: 10.1016/0165-022x(86)90090-4.
Inhibition of microsomal NAD(P)H oxidation by Triton X-100
- PMID: 3465799
- DOI: 10.1016/0165-022x(86)90090-4
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Inhibition of microsomal NAD(P)H oxidation by Triton X-100
J Biochem Biophys Methods.
1986 Oct.
Abstract
The non-ionic detergent Triton X-100 is shown to inhibit the spontaneous oxidation of NAD(P)H associated with rat liver microsomes. Advantage of this observation is taken to measure different microsomal NAD(P)H-dependent oxidoreductase activities such as 3-alpha-hydroxysteroid dehydrogenase, dihydrodiol dehydrogenase and various xenobiotic oxidoreductases. This inhibition provides an easy method for the screening of the under-investigated microsomal oxidoreductive metabolism of xenobiotics.
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