Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1986 Oct;13(3):171-8.
doi: 10.1016/0165-022x(86)90090-4.

Inhibition of microsomal NAD(P)H oxidation by Triton X-100

J A Boutin

Inhibition of microsomal NAD(P)H oxidation by Triton X-100

J A Boutin. J Biochem Biophys Methods. 1986 Oct.

Abstract

The non-ionic detergent Triton X-100 is shown to inhibit the spontaneous oxidation of NAD(P)H associated with rat liver microsomes. Advantage of this observation is taken to measure different microsomal NAD(P)H-dependent oxidoreductase activities such as 3-alpha-hydroxysteroid dehydrogenase, dihydrodiol dehydrogenase and various xenobiotic oxidoreductases. This inhibition provides an easy method for the screening of the under-investigated microsomal oxidoreductive metabolism of xenobiotics.

PubMed Disclaimer

Publication types

MeSH terms

LinkOut - more resources