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. 2021 Oct 1:12:727734.
doi: 10.3389/fpls.2021.727734. eCollection 2021.

Molecular Cytogenetic Identification of a Novel Wheat- Thinopyrum ponticum 1JS (1B) Substitution Line Resistant to Powdery Mildew and Leaf Rust

Mingzhu Li  1   2 , Yanzhen Wang  3 , Xiaojuan Liu  1   2 , Xingfeng Li  1   2 , Honggang Wang  1   2 , Yinguang Bao  1   2
Affiliations

Molecular Cytogenetic Identification of a Novel Wheat- Thinopyrum ponticum 1JS (1B) Substitution Line Resistant to Powdery Mildew and Leaf Rust

Mingzhu Li et al. Front Plant Sci. .

Abstract

Thinopyrum ponticum (2n = 10x = 70) is a wild relative of wheat with high tolerance to both biotic and abiotic stresses; it has been wildly used in wheat genetic improvement. A disomic substitution line named SN19647 was derived from a cross between Triticum aestivum and the wheat-Th. ponticum partial amphiploid SNTE20 (2n = 8x = 56). It was evaluated for disease resistance and characterized via sequential fluorescence in situ hybridization (FISH)-genomic in situ hybridization (GISH) and molecular markers. The results showed that SN19647 carried resistance to both powdery mildew and leaf rust. It contained 42 chromosomes with a pair of wheat chromosome 1B replaced by a pair of JS chromosomes from Th. ponticum. In addition to chromosomal substitution events, structural variation also occurred on wheat chromosomes 2A, 5A, 6B, and 7B. Based on marker analysis, 19 markers specific to the JS chromosome were obtained, of which seventeen markers belonged to homoeologous group one. These results indicated that SN19647 was a 1JS (1B) substitution line. Compared with the known 1JS (1D) substitution line CH10A5, it was found that 17 markers generated different specific bands to Th. ponticum, confirming the novelty of the 1JS chromosome in SN19647. Therefore, SN19647, resistant to powdery mildew and leaf rust, was a novel 1JS (1B) substitution line that can be used in wheat genetic improvement.

Keywords: FISH; GISH; Thinopyrum ponticum; leaf rust; powdery mildew; substitution line.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
Reactions to powdery mildew and leaf rust. (A) Reactions to powdery mildew at the seedling stage. (B) Reactions to powdery mildew at the adult stage. (C) Reactions to leaf rust at the adult stage. 1–7 refers to Th. ponticum, YN15, SNF63, SNTE20, JM22, SN19647 and HXH, respectively.
Figure 2
Figure 2
In situ hybridization analysis of SN19647. (A) GISH pattern of SN19647 probed with Th. ponticum genomic DNA. (B) GISH pattern of SN19647 probed with Ps. spicata genomic DNA. (C) FISH pattern of SN19647. (D) Comparison of wheat chromosomes in SNTE20 (a), JM22 (b) and SN19647 (c). Arrows indicate the chromosomes JS or locations with different FISH bands. Scale bars = 10 μm.
Figure 3
Figure 3
Molecular marker analysis of SN19647. (A) CINAU851. (B) CINAU857. (C) CINAU859. (D) CINAU862. (E) CINAU865. (F) CINAU866. (G) CINAU870. (H) CINAU880. (I) CINAU889. (J) CINAU890. (K) CINAU971. (L) CINAU1616. (M) Xgwm268. (N) SWES650. (O) Xmag3030. (P) BE405232. Lanes: M, DL2000 marker; 1-4 refer to Th. ponticum, SNTE20, JM22, and SN19647, respectively. Arrows indicate the specific bands of Th. ponticum.
Figure 4
Figure 4
Molecular marker analysis of SN19747 and CH10A5 (specific markers in CH10A5). (A) Xcfd63. (B) TNAC1044-HaeIII. (C) Xwmc432. (D) Xwmc93. (E) HaeIII-TNAC1088. (F) TNAC1021-TaqI. (G) BG313767. (H) TNAC1026-HaeIII. Lanes: M, DL2000; 1-7 refer to Th. ponticum, SNTE20, SNF63, YN15, JM22, SN19647 and CH10A5, respectively. The blue, red and white arrows indicate the bands shared between SN19647 and CH10A5, specific to CH10A5 and SN19647, respectively.
Figure 5
Figure 5
Molecular marker analysis of SN19647 and CH10A5 (specific markers in SN19647). (A) CINAU857. (B) CINAU889. (C) CINAU886. (D) CINAU862. (E) CINAU880. (F) CINAU851. (G) CINAU859. (H) CINAU865. (I) CINAU870. (J) CINAU890. (K) SWES650. (L) Xgwm268. (M) BE405232. (N) Xmag3030. Lanes: M, DL2000; 1-7 refer to Th. ponticum, SNTE20, SNF63, YN15, JM22, SN19647 and CH10A5, respectively. The blue, red, and white arrows indicate the band shared between SN19647 and CH10A5, specific to CH10A5 and SN19647, respectively.
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