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. 2021 Oct 18;11(10):e050296.
doi: 10.1136/bmjopen-2021-050296.

Role and utility of COVID-19 laboratory testing in low-income and middle-income countries: protocol for rapid evidence synthesis

Ojiambo Kevin Ouma  1   2 , Kisangala Ephraim  2   3 , Nakalembe Loyce  2   4 , Eve Namisango  2   5 , Fred Nalugoda  2   6 , Regina Ndagire  2   3 , Rachel Nante Wangi  2   3 , Brenda Allen Kawala  2   7 , Thomas Katairo  2   8 , Allen Eva Okullo  2   9 , Robert Apunyo  2 , Daniel Semakula  2   10 , Ash Luwambo  2   11 , Alison Annet Kinengyere  2   12 , Nelson Sewankambo  2   13 , Sheila N Balinda  14 , Moses Ocan  15   16 , Ekwaro A Obuku  2   17
Affiliations

Role and utility of COVID-19 laboratory testing in low-income and middle-income countries: protocol for rapid evidence synthesis

Ojiambo Kevin Ouma et al. BMJ Open. .

Abstract

Introduction: Accurate and affordable laboratory testing is key to timely diagnosis and appropriate management of patients with COVID-19. New laboratory test protocols are released into the market under emergency use authorisation with limited evidence on diagnostic test accuracy. As such, robust evidence on the diagnostic accuracy and the costs of available tests is urgently needed to inform policy and practice especially in resource-limited settings. We aim to determine the diagnostic test accuracy, cost-effectiveness and utility of laboratory test strategies for COVID-19 in low-income and middle-income countries.

Methods and analysis: This will be a multistaged, protocol-driven systematic review conducted in line with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines for diagnostic test accuracy studies. We will search for relevant literature in at least six public health databases, including PubMed, Google Scholar, MEDLINE, Scopus, Web of Science and the WHO Global Index Medicus. In addition, we will search Cochrane Library, COVID-END and grey literature databases to identify additional relevant articles before double-screening and abstraction of data. We will conduct a structured narrative and quantitative synthesis of the results guided by the Fryback and Thornbury framework for assessing a diagnostic test. The primary outcome is COVID-19 diagnostic test accuracy. Using the GRADE approach specific to diagnostic accuracy tests, we will appraise the overall quality of evidence and report the results following the original PRISMA statement. The protocol is registered with the International Prospective Register of Systematic Reviews (PROSPERO; https://www.crd.york.ac.uk/prospero/).

Ethics and dissemination: Ethical review was done by the School of Biomedical Sciences Research Ethics Committee and the Uganda National Council for Science and Technology. The published article will be accessible to policy and decision makers. The findings of this review will guide clinical practice and policy decisions and highlight areas for future research.PROSPERO registration number CRD42020209528.

Keywords: COVID-19; infectious diseases; infectious diseases & infestations; microbiology; protocols & guidelines.

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Conflict of interest statement

Competing interests: None declared.

References

    1. World Health Organization . Diagnostic testing for SARS-CoV-2: interim guidance, 2020. Available: https://www.who.int/publications/i/item/diagnostic-testing-for-sars-cov-2 [Accessed 11 Sept 2020].
    1. World Health Organization . Timeline of WHO’s response to COVID-19, 2020. Available: https://www.who.int/emergencies/diseases/novel-coronavirus-2019/interact...
    1. World Health Organization . Laboratory testing for coronavirus disease 2019 (‎COVID-19)‎ in suspected human cases: interim guidance, 2 March 2020, 2020. Available: https://apps.who.int/iris/handle/10665/331329
    1. Tahamtan A, Ardebili A. Real-time RT-PCR in COVID-19 detection: issues affecting the results. Expert Rev Mol Diagn 2020;20:453–4. 10.1080/14737159.2020.1757437 - DOI - PMC - PubMed
    1. Yan M, Zheng Y, Sun Y, et al. . Analysis of the diagnostic value of serum specific antibody testing for coronavirus disease 2019. J Med Virol 2021;93:441–7. 10.1002/jmv.26230 - DOI - PMC - PubMed

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